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Protein Domain : IPR007624

Name  RNA polymerase sigma-70 region 3 Short Name  RNA_pol_sigma70_r3
Type  Domain Description  The bacterial core RNA polymerase complex, which consists of five subunits, is sufficient for transcription elongation and termination but is unable to initiate transcription. Transcription initiation from promoter elements requires a sixth, dissociable subunit called a sigma factor, which reversibly associates with the core RNA polymerase complex to form a holoenzyme []. RNA polymerase recruits alternative sigma factors as a means of switching on specific regulons. Most bacteria express a multiplicity of sigma factors. Two of these factors, sigma-70 (gene rpoD), generally known as the major or primary sigma factor, and sigma-54 (gene rpoN or ntrA) direct the transcription of a wide variety of genes. The other sigma factors, known as alternative sigma factors, are required for the transcription of specific subsets of genes.With regard to sequence similarity, sigma factors can be grouped into two classes, the sigma-54 and sigma-70 families. Sequence alignments of the sigma70 family members reveal four conserved regions that can be further divided into subregions eg. sub-region 2.2, which may be involved in the binding of the sigma factor to the core RNA polymerase; and sub-region 4.2, which seems to harbor a DNA-binding 'helix-turn-helix' motif involved in binding the conserved -35 region of promoters recognised by the major sigma factors [, ]. Region 3 forms a discrete compact three helical domain within the sigma-factor. Region is not normally involved in the recognition of promoter DNA, but in some specific bacterial promoters containing an extended -10 promoter element, residues within region 3 play an important role. Region 3 primarily is involved in binding the core RNA polymerase in the holoenzyme [].

Publication Counts Displayer

0 Child Features

0 Contains

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF04539 PFAM IPR007624

2 Data Sets

Name URL
InterPro data set  
InterPro GO Annotation data set  

13 Found In

DB identifier Name Short Name Type
IPR011991 Winged helix-turn-helix DNA-binding domain WHTH_DNA-bd_dom Domain
IPR013324 RNA polymerase sigma factor, region 3/4 RNA_pol_sigma_r3_r4 Domain
IPR014284 RNA polymerase sigma-70 like domain RNA_pol_sigma-70_dom Domain
IPR012761 RNA polymerase sigma factor RpoS RNA_pol_sigma_RpoS Family
IPR016262 RNA polymerase sigma factor, SigB/SigC/SigD, plastid RNA_pol_sigma_SigB/C/D_plastid Family
IPR017848 RNA polymerase sigma factor, RpoD-like, cyanobacteria RNA_pol_sigma_RpoD_cyanobac Family
IPR014212 RNApolymerase sigma-G type RNA_pol_sigma-G Family
IPR014236 RNA polymerase sigma-F type RNA_pol_sigma-F Family
IPR014288 RNA polymerase sigma-B type RNA_pol_sigma-B Family
IPR012845 RNA polymerase sigma factor, FliA/WhiG RNA_pol_sigma_FliA_WhiG Family
IPR014322 RNA polymerase sigma-B/F/G type RNA_pol_sigma-B/F/G Family
IPR000943 RNA polymerase sigma-70 RNA_pol_sigma70 Domain
IPR012760 RNA polymerase sigma factor RpoD, C-terminal RNA_pol_sigma_RpoD_C Domain

5 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
RNA polymerase sigma-70 region 3   GO:0003677 DNA binding
RNA polymerase sigma-70 region 3   GO:0003700 sequence-specific DNA binding transcription factor activity
RNA polymerase sigma-70 region 3   GO:0016987 sigma factor activity
RNA polymerase sigma-70 region 3   GO:0006352 DNA-templated transcription, initiation
RNA polymerase sigma-70 region 3   GO:0006355 regulation of transcription, DNA-templated

5 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
RNA polymerase sigma-70 region 3   GO:0003677 DNA binding
RNA polymerase sigma-70 region 3   GO:0003700 sequence-specific DNA binding transcription factor activity
RNA polymerase sigma-70 region 3   GO:0016987 sigma factor activity
RNA polymerase sigma-70 region 3   GO:0006352 DNA-templated transcription, initiation
RNA polymerase sigma-70 region 3   GO:0006355 regulation of transcription, DNA-templated

0 Parent Features

0 Proteins