modENCODE |  Help |  blog

Protein Domain : IPR004409

Name  Biotin operon repressor, helix-turn-helix domain Short Name  Biotin_operon_repress_HTH
Type  Domain Description  The biotin operon of Escherichia colicontains 5 structural genes involved in the synthesis of biotin. Transcription of the operon is regulated via one of these proteins, BirA. BirA is an asymetric protein with 3 specific domains. The ligase reaction intermediate, biotinyl-5'-AMP, is the co-repressor that triggers DNA binding by BirA.The alpha-helical N-terminal domain of the BirA protein has the helix-turn-helix structure of DNA-binding proteins with a central DNA recognition helix. BirA undergoes several conformational changes related to repressor function and the N-terminal DNA-binding function is connected to the rest of the molecule through a hinge which will allow relocation of the domains during the reaction []. Two repressor molecules form the operator-repressor complex, with dimer formation occuring simultaneously with DNA binding. DNA-binding may cause a conformational change which allows this co-operative interaction. In the dimer structure, the beta-sheets in the central domain of each monomer are arranged side-by-side to form a single, seamless beta-sheet.

Publication Counts Displayer

0 Child Features

0 Contains

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
TIGR00122 TIGRFAMs IPR004409

2 Data Sets

Name URL
InterPro data set  
InterPro GO Annotation data set  

0 Found In

1 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Biotin operon repressor, helix-turn-helix domain   GO:0006355 regulation of transcription, DNA-templated

1 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Biotin operon repressor, helix-turn-helix domain   GO:0006355 regulation of transcription, DNA-templated

1 Parent Features

DB identifier Name Short Name Type
IPR011991 Winged helix-turn-helix DNA-binding domain WHTH_DNA-bd_dom Domain

0 Proteins