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Protein Domain : IPR000485

Name  AsnC-type HTH domain Short Name  AsnC-type_HTH_dom
Type  Domain Description  The asnC-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 60 amino acids present in transcription regulators of the asnC/lrp family. This family of prokaryotic regulators is named after Escherichia coli asnC and Leucine-responsive Regulatory Protein (lrp), which are a regulator of asparagine synthesis and a global regulator of various operons, respectively []. AsnC/lrp-like proteins are present in bacteria and archaea []. The DNA-binding asnC-type HTH domain occurs usually in the N-terminal part. The C-terminal part can contain an effector-binding domain and/or an oligomerisation domain. The crystal structure of hyperthermophilic archaeallrpA shows that the N-terminal, DNA binding domain contains a core of three alpha-helices, followed by a single beta-strand, which connects as a flexible hinge to the effector binding domain. The second and third helices, connected via a turn, comprise the helix-turn-helix motif. Helix 3 is termed the recognition helix as it binds the DNA major groove, like in other HTHs. Most E. coli lrp DNA binding mutants are positioned in the lrpA structure onthe HTH and three are on the hinge [].Proteins known to contain an asnC-type HTH domain include:Escherichia coli Leucine-responsive Regulatory Protein (lrp), a global transcriptional regulator of 35-75 different genes involved in amino acid biosynthesis, amino acid degradation, transport or pili formation. Binding of leucine by lrp can stimulate or reduce the regulatory effect of activation for some operons or repression for others. Lrp negatively autoregulates the lrp gene, independently of leucine.Salmonella typhimurium lrp, a global leucine-responsive regulator involved in branched-chain amino acid biosynthesis, pili formation and plasmid virulence.Escherichia coli asnC, a specific asparagine-dependent transcriptional activator of asparagine biosynthesis. AsnC is also an asparagine-independent repressor of its own transcription.Pseudomonas putida bkdR, a specific autoregulatory transcriptional regulator, involved in catabolism of branched-chain amino acids.Agrobacterium tumefaciens putR, a specific proline-responsive regulator of proline catabolism.Bacillus subtilis lrpA/lrpB and lrpC, transcriptional regulators involved in serine-glycine interconversion, sporulation and amino acid metabolism. LrpC binds to a specific DNA structure and wraps and overwinds the DNA [].Bacillus subtilis azlB, a specific transcriptional repressor of branched-chain amino acid transport.Pyrococcus furiosus lrpA, a putative lrp with negative autoregulation.Zymomonas mobilis grp, a repressor of the glutamate uptake operon.

Publication Counts Displayer

0 Child Features

1 Contains

DB identifier Name Short Name Type
IPR019885 Transcription regulator HTH, AsnC-type, conserved site Tscrpt_reg_HTH_AsnC-type_CS Conserved_site

3 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF13404 PFAM IPR000485
PR00033 PRINTS IPR000485
PS50956 PROFILE IPR000485

2 Data Sets

Name URL
InterPro data set  
InterPro GO Annotation data set  

1 Found In

DB identifier Name Short Name Type
IPR019888 Transcription regulator AsnC-type Tscrpt_reg_AsnC-typ Family

2 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
AsnC-type HTH domain   GO:0043565 sequence-specific DNA binding
AsnC-type HTH domain   GO:0006355 regulation of transcription, DNA-templated

2 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
AsnC-type HTH domain   GO:0043565 sequence-specific DNA binding
AsnC-type HTH domain   GO:0006355 regulation of transcription, DNA-templated

1 Parent Features

DB identifier Name Short Name Type
IPR011991 Winged helix-turn-helix DNA-binding domain WHTH_DNA-bd_dom Domain

0 Proteins