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Protein Domain : IPR013037

Name  Clathrin adaptor, beta-adaptin, appendage, Ig-like subdomain Short Name  Clathrin_b-adaptin_app_Ig-like
Type  Domain Description  Proteins synthesized on the ribosome and processed in the endoplasmic reticulum are transported from the Golgi apparatus to the trans-Golgi network (TGN), and from there via small carrier vesicles to their final destination compartment. These vesicles have specific coat proteins (such as clathrin or coatomer) that are important for cargo selection and direction of transport []. Clathrin coats contain both clathrin (acts as a scaffold) and adaptor complexes that link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The two major types of clathrin adaptor complexes are the heterotetrameric adaptor protein (AP) complexes, and the monomeric GGA (Golgi-localising, Gamma-adaptin ear domain homology, ARF-binding proteins) adaptors [, ].AP (adaptor protein) complexes are found in coated vesicles and clathrin-coated pits. AP complexes connect cargo proteins and lipids to clathrin at vesicle budding sites, as well as binding accessory proteins that regulate coat assembly and disassembly (such as AP180, epsins and auxilin). There are different AP complexes in mammals. AP1 is responsible for the transport of lysosomal hydrolases between the TGN and endosomes []. AP2 associates with the plasma membrane and is responsible for endocytosis []. AP3 is responsible for protein trafficking to lysosomes and other related organelles []. AP4 is less well characterised. AP complexes are heterotetramers composed of two large subunits (adaptins), a medium subunit (mu) and a small subunit (sigma). For example, in AP1 these subunits are gamma-1-adaptin, beta-1-adaptin, mu-1 and sigma-1, while in AP2 they are alpha-adaptin, beta-2-adaptin, mu-2 and sigma-2. Each subunit has a specific function. Adaptins recognise and bind to clathrin through their hinge region (clathrin box), and recruit accessory proteins that modulate AP function through their C-terminal ear (appendage) domains. Mu recognises tyrosine-based sorting signals within the cytoplasmic domains of transmembrane cargo proteins []. One function of clathrin and AP2 complex-mediated endocytosis is to regulate the number of GABA(A) receptors available at the cell surface []. This entry represents a beta-sandwich structural motif found in the appendage (ear) domain of gamma1-adaptin from AP1 clathrin adaptor complex, and the homologous C-terminal GAE (gamma-adaptin ear) domain of GGA adaptor proteins. These domains have an immunoglobulin-like beta-sandwich fold containing 8 strands in 2 beta-sheets in a Greek key topology [, ]. This is a similar fold to that found in alpha- and beta-adaptins, but there is little sequence identity between them. The GAE domain is involved in the recruitment of accessory proteins, such as gamma-synergin, Rababptin-5, Eps15 and cyclin G-associated kinase, which modulate the functions of GAE domain containing proteins in the membrane trafficking events [, ]. The binding site in GAE for accessory proteins is located in a shallow hydrophobic trough surrounded by charged (mainly basic) residues [].More information about these proteins can be found at Protein of the Month: Clathrin [].

Publication Counts Displayer

0 Child Features

1 Contains

DB identifier Name Short Name Type
IPR008152 Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain Clathrin_a/b/g-adaptin_app_Ig Domain

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
G3DSA:2.60.40.1150 GENE3D IPR013037

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

1 Found In

DB identifier Name Short Name Type
IPR016342 AP-1, 2,4 complex subunit beta AP_complex_bsu_1_2_4 Family

3 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Clathrin adaptor, beta-adaptin, appendage, Ig-like subdomain   GO:0006886 intracellular protein transport
Clathrin adaptor, beta-adaptin, appendage, Ig-like subdomain   GO:0016192 vesicle-mediated transport
Clathrin adaptor, beta-adaptin, appendage, Ig-like subdomain   GO:0030131 clathrin adaptor complex

3 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Clathrin adaptor, beta-adaptin, appendage, Ig-like subdomain   GO:0006886 intracellular protein transport
Clathrin adaptor, beta-adaptin, appendage, Ig-like subdomain   GO:0016192 vesicle-mediated transport
Clathrin adaptor, beta-adaptin, appendage, Ig-like subdomain   GO:0030131 clathrin adaptor complex

1 Parent Features

DB identifier Name Short Name Type
IPR013041 Coatomer/clathrin adaptor appendage, Ig-like subdomain Coatomer/clathrin_app_Ig-like Domain

11 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0074558 Q24253 Drosophila melanogaster
FBpp0272566 Q29H04 Drosophila pseudoobscura
FBpp0165315 B4L6V2 Drosophila mojavensis
FBpp0230163 B4MA28 Drosophila virilis
FBpp0260880 B4PZ07 Drosophila yakuba
A8X9Q7_CAEBR A8X9Q7 Caenorhabditis briggsae
G0MKM1_CAEBE G0MKM1 Caenorhabditis brenneri
Q7YZG8_CAEEL Q7YZG8 Caenorhabditis elegans
Q9N4F3_CAEEL Q9N4F3 Caenorhabditis elegans
Q960F2_DROME Q960F2 Drosophila melanogaster
E3LRV2_CAERE E3LRV2 Caenorhabditis remanei