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Protein Domain : IPR015721

Name  Rho GTP exchange factor Short Name  RhoGEF-like
Type  Family Description  The Rho family GTPases Rho, Rac and CDC42 regulate a diverse array of cellular processes, and are critical regulators actin cytoskeleton dynamics. Like all members of the Ras superfamily, the Rho proteins cycle between active GTP-bound and inactive GDP-bound conformational states. Activation of Rho proteins through release of bound GDP and subsequent binding of GTP, is catalyzed by guanine nucleotide exchange factors (GEFs) in the Dbl family. The proteins encoded by members of the Dbl family share a common domain, presented in this entry, of about 200 residues (designated the Dbl homology or DH domain) that has been shown to encode a GEF activity specific for a number of Rho family members. In addition, all family members possess a second, shared domain designated the pleckstrin homology (PH) domain (). The PH domain is invariably located immediately C-terminal to the DH domain and this invariant topography suggests a functional interdependence between these two structural modules. Biochemical data have established the role of the conserved DH domain in Rho GTPase interaction and activation, and the role of the tandem PH domain in intracellular targeting and/or regulation of DH domain function. The DH domain of Dbl has been shown to mediate oligomerisation that is mostly homophilic in nature. In addition to the tandem DH/PH domains Dbl family GEFs contain diverse structural motifs like serine/threonine kinase, RBD, PDZ, RGS, IQ, REM, Cdc25 RasGEF, CH, SH2, SH3, EF, spectrin or Ig.The DH domain is composed of three structurally conserved regions separated by more variable regions. It does not share significant sequence homology with other subtypes of small G-protein GEF motifs such as the Cdc25 domain and the Sec7 domain, which specifically interact with Ras and ARF family small GTPases, respectively, nor with other Rho protein interactive motifs, indicating that the Dbl family proteins are evolutionarily unique. The DH domain is composed of 11 alpha-helices that are folded into a flattened, elongated alpha-helix bundle in which two of the three conserved regions, conserved region 1 (CR1) and conserved region 3 (CR3), are exposed near the centre of one surface. CR1 and CR3, together with a part of alpha-6 and the DH/PH junction site, constitute the Rho GTPase interacting pocket.

Publication Counts Displayer

0 Child Features

4 Contains

DB identifier Name Short Name Type
IPR001849 Pleckstrin homology domain Pleckstrin_homology Domain
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol binding Prot_Kinase_C-like_PE/DAG-bd Domain
IPR000219 Dbl homology (DH) domain DH-domain Domain
IPR015212 Regulator of G protein signalling-like domain RGS-like_dom Domain

1 Cross References

Source . Name

Subject . Primary Identifier

2 Data Sets

Name URL
TrEMBL data set
InterPro data set  

0 Found In

0 GO Annotation

0 Ontology Annotations

0 Parent Features

36 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0086123 A1ZAN7 Drosophila melanogaster
FBpp0086124 A1ZAN6 Drosophila melanogaster
FBpp0080821 Q9VIV0 Drosophila melanogaster
FBpp0302998 M9PBE1 Drosophila melanogaster
FBpp0287994 Q29K62 Drosophila pseudoobscura
FBpp0276614 B5E0Q5 Drosophila pseudoobscura
FBpp0168986 B4KIW6 Drosophila mojavensis
FBpp0170556 B4KML9 Drosophila mojavensis
FBpp0232511 B4M8K1 Drosophila virilis
FBpp0234102 B4MCK8 Drosophila virilis
FBpp0256648 B4P8I4 Drosophila yakuba
FBpp0257656 B4PAZ9 Drosophila yakuba
A8WXP4_CAEBR A8WXP4 Caenorhabditis briggsae
A8WZ74_CAEBR A8WZ74 Caenorhabditis briggsae
H2W7J5_CAEJA H2W7J5 Caenorhabditis japonica
H2WQV1_CAEJA H2WQV1 Caenorhabditis japonica
K7IE95_CAEJA K7IE95 Caenorhabditis japonica
K7I924_CAEJA K7I924 Caenorhabditis japonica
K7I923_CAEJA K7I923 Caenorhabditis japonica
G0NV88_CAEBE G0NV88 Caenorhabditis brenneri
G0MI70_CAEBE G0MI70 Caenorhabditis brenneri
G5EFE1_CAEEL G5EFE1 Caenorhabditis elegans
H2L0P5_CAEEL H2L0P5 Caenorhabditis elegans
H2L0P4_CAEEL H2L0P4 Caenorhabditis elegans
H2L0P6_CAEEL H2L0P6 Caenorhabditis elegans
Q9N2V4_CAEEL Q9N2V4 Caenorhabditis elegans
H2L0P8_CAEEL H2L0P8 Caenorhabditis elegans
H2L0P7_CAEEL H2L0P7 Caenorhabditis elegans
Q6T5A2_CAEEL Q6T5A2 Caenorhabditis elegans
Q8T9E3_DROME Q8T9E3 Drosophila melanogaster