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Protein Domain : IPR017919

Name  Transcription factor TFE/TFIIEalpha HTH domain Short Name  TFIIE/TFIIEa_HTH
Type  Domain Description  Initiation of eukaryotic mRNA transcription requires melting of promoter DNA with the help of the general transcription factors TFIIE and TFIIH. In higher eukaryotes, the general transcription factor TFIIE consists of two subunits: the large alpha subunit () and the small beta (). TFIIE beta has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. The approximately 120-residue central core domain of TFIIE beta plays a role in double-stranded DNA binding of TFIIE [].The TFIIE beta central core DNA-binding domain consists of three helices with a beta hairpin at the C terminus, resembling the winged helix proteins. It shows a novel double-stranded DNA-binding activity where the DNA-binding surface locates on the opposite side to the previously reported winged helix motif by forming a positively charged furrow [].Archaea contain a TFIIE homolog, called TFE, which corresponds to the N-terminal half of TFIIEalpha. It appears that archaeal TFE corresponds to the minimal essential region of eukaryotic TFIIEalpha. In archaea TFE contains an N-terminal, weakly conserved, helix-turn-helix (HTH) motif within a leucine-rich region and a C-terminal zinc ribbon [, , ]. It has been proposed that the TFE/IIEalpha-type HTH domain acts as a bridging factor or adapter between the TATA box-binding protein, the polymerase, and possibly promoter DNA [].The TFE/IIEalpha-type HTH domain adopts a winged HTH (winged helix) fold, comprising three alpha-helices and three beta-strands in the canonical order alpha1-beta1-alpha2-alpha3-beta2-beta3. Conserved residues within helices alpha1-alpha3 form the tightly packed hydrophobic core of the winged helix domain. A specific feature of the structure is the extension of the canonical winged helix fold at the N and C termini by the additional helices alpha0 and alpha4, respectively. Hydrophobic residues from the additional helix alpha0 extend the hydrophobic core of the winged helix domain, and helix alpha0 is tightly packed against the canonical winged helix fold. Helix alpha4 comprises only one turn [].

Publication Counts Displayer

0 Child Features

0 Contains

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PS51344 PROFILE IPR017919

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

3 Found In

DB identifier Name Short Name Type
IPR011991 Winged helix-turn-helix DNA-binding domain WHTH_DNA-bd_dom Domain
IPR002853 Transcription factor TFIIE, alpha subunit TFIIE_asu Family
IPR016481 Transcription factor TFE, archaea TF_E_archaea Family

1 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Transcription factor TFE/TFIIEalpha HTH domain   GO:0043565 sequence-specific DNA binding

1 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Transcription factor TFE/TFIIEalpha HTH domain   GO:0043565 sequence-specific DNA binding

1 Parent Features

DB identifier Name Short Name Type
IPR024550 TFIIEalpha/SarR/Rpc3 HTH domain TFIIEa/SarR/Rpc3_HTH_dom Domain

9 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0075839 O96880 Drosophila melanogaster
FBpp0274182 Q2LZ72 Drosophila pseudoobscura
FBpp0162934 B4KUS0 Drosophila mojavensis
FBpp0228477 B4LCQ5 Drosophila virilis
FBpp0266846 B4PFM4 Drosophila yakuba
A8WMX3_CAEBR A8WMX3 Caenorhabditis briggsae
G0MRB8_CAEBE G0MRB8 Caenorhabditis brenneri
G5EG49_CAEEL G5EG49 Caenorhabditis elegans
E3NBB7_CAERE E3NBB7 Caenorhabditis remanei