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Protein Domain : IPR009028

Name  Coatomer/calthrin adaptor appendage, C-terminal subdomain Short Name  Coatomer/calthrin_app_sub_C
Type  Domain Description  Proteins synthesized on the ribosome and processed in the endoplasmic reticulum are transported from the Golgi apparatus to the trans-Golgi network (TGN), and from there via small carrier vesicles to their final destination compartment. This traffic is bidirectional, to ensure that proteins required to form vesicles are recycled. Vesicles have specific coat proteins (such as clathrin or coatomer) that are important for cargo selection and direction of transfer []. Clathrin coats contain both clathrin and adaptor complexes that link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The two major types of clathrin adaptor complexes are the heterotetrameric adaptor protein (AP) complexes, and the monomeric GGA (Golgi-localising, Gamma-adaptin ear domain homology, ARF-binding proteins) adaptors []. All AP complexes are heterotetramers composed of two large subunits (adaptins), a medium subunit (mu) and a small subunit (sigma). Each subunit has a specific function. Adaptin subunits recognise and bind to clathrin through their hinge region (clathrin box), and recruit accessory proteins that modulate AP function through their C-terminal appendage domains. By contrast, GGAs are monomers composed of four domains, which have functions similar to AP subunits: an N-terminal VHS (Vps27p/Hrs/Stam) domain, a GAT (GGA and Tom1) domain, a hinge region, and a C-terminal GAE (gamma-adaptin ear) domain. The GAE domain is similar to the AP gamma-adaptin ear domain, being responsible for the recruitment of accessory proteins that regulate clathrin-mediated endocytosis [].While clathrin mediates endocytic protein transport from ER to Golgi, coatomers (COPI, COPII) primarily mediate intra-Golgi transport, as well as the reverse Golgi to ER transport of dilysine-tagged proteins []. Coatomers reversibly associate with Golgi (non-clathrin-coated) vesicles to mediate protein transport and for budding from Golgi membranes []. Coatomer complexes are hetero-oligomers composed of at least an alpha, beta, beta', gamma, delta, epsilon and zeta subunits. The alpha and beta2 adaptor subunits can each be divided into a trunk domain and the appendage domain (or ear domain), separated by a linker region. Clathrin polymerisation is promoted by its binding to the beta2 appendage and hinge domains. The alpha appendage domain interacts with a number of accessory proteins, including eps15, epsin, amphiphysin, AP180, auxilin, numb, and Dab2, thereby regulating the translocation of these proteins to the bud site. This entry represents a subdomain of the appendage (ear) domain of alpha- and beta-adaptin from AP clathrin adaptor complexes, and the appendage domain of the gamma subunit of coatomer complexes. These domains have a three-layer arrangement, alpha-beta-alpha, with a bifurcated antiparallel beta-sheet [, , []. Although the appendage domains from AP adaptins and coatomers share a similar fold, there is little sequence identity between them. However, they also share similar motif-based cargo recognition and accessory factor recruitment mechanisms. More information about these proteins can be found at Protein of the Month: Clathrin [].

Publication Counts Displayer

1 Child Features

DB identifier Name Short Name Type
IPR015873 Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain Clathrin_a/coatomer_app_sub_C Domain

0 Contains

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
SSF55711 SSF IPR009028

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

0 Found In

3 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Coatomer/calthrin adaptor appendage, C-terminal subdomain   GO:0006886 intracellular protein transport
Coatomer/calthrin adaptor appendage, C-terminal subdomain   GO:0016192 vesicle-mediated transport
Coatomer/calthrin adaptor appendage, C-terminal subdomain   GO:0030117 membrane coat

3 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Coatomer/calthrin adaptor appendage, C-terminal subdomain   GO:0006886 intracellular protein transport
Coatomer/calthrin adaptor appendage, C-terminal subdomain   GO:0016192 vesicle-mediated transport
Coatomer/calthrin adaptor appendage, C-terminal subdomain   GO:0030117 membrane coat

0 Parent Features

44 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0085155 Q8I0G5 Drosophila melanogaster
FBpp0074558 Q24253 Drosophila melanogaster
FBpp0088945 E8NH12 Drosophila melanogaster
FBpp0088490 P91926 Drosophila melanogaster
FBpp0272566 Q29H04 Drosophila pseudoobscura
FBpp0280380 Q29N38 Drosophila pseudoobscura
FBpp0297038 I5ANE0 Drosophila pseudoobscura
FBpp0282417 Q29AE5 Drosophila pseudoobscura
FBpp0165315 B4L6V2 Drosophila mojavensis
FBpp0167254 B4KFJ2 Drosophila mojavensis
FBpp0172613 B4K7Z2 Drosophila mojavensis
FBpp0230163 B4MA28 Drosophila virilis
FBpp0234164 B4LTK9 Drosophila virilis
FBpp0237503 B4M106 Drosophila virilis
FBpp0255956 B4PNE7 Drosophila yakuba
FBpp0260880 B4PZ07 Drosophila yakuba
FBpp0261149 B4P2D9 Drosophila yakuba
A8XNZ9_CAEBR A8XNZ9 Caenorhabditis briggsae
A8WUZ6_CAEBR A8WUZ6 Caenorhabditis briggsae
A8X9Q7_CAEBR A8X9Q7 Caenorhabditis briggsae
K7GXM2_CAEJA K7GXM2 Caenorhabditis japonica
K7I3S0_CAEJA K7I3S0 Caenorhabditis japonica
K7GXM1_CAEJA K7GXM1 Caenorhabditis japonica
H2WQI4_CAEJA H2WQI4 Caenorhabditis japonica
H2W9N4_CAEJA H2W9N4 Caenorhabditis japonica
G0MKM1_CAEBE G0MKM1 Caenorhabditis brenneri
G0NUQ5_CAEBE G0NUQ5 Caenorhabditis brenneri
G0NBB6_CAEBE G0NBB6 Caenorhabditis brenneri
G0MGV3_CAEBE G0MGV3 Caenorhabditis brenneri
G0PIP7_CAEBE G0PIP7 Caenorhabditis brenneri