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Protein Domain : IPR015873

Name  Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain Short Name  Clathrin_a/coatomer_app_sub_C
Type  Domain Description  Proteins synthesized on the ribosome and processed in the endoplasmic reticulum are transported from the Golgi apparatus to the trans-Golgi network (TGN), and from there via small carrier vesicles to their final destination compartment. This traffic is bidirectional, to ensure that proteins required to form vesicles are recycled. Vesicles have specific coat proteins (such as clathrin or coatomer) that are important for cargo selection and direction of transfer []. Clathrin coats contain both clathrin and adaptor complexes that link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The two major types of clathrin adaptor complexes are the heterotetrameric adaptor protein (AP) complexes, and the monomeric GGA (Golgi-localising, Gamma-adaptin ear domain homology, ARF-binding proteins) adaptors []. All AP complexes are heterotetramers composed of two large subunits (adaptins), a medium subunit (mu) and a small subunit (sigma). Each subunit has a specific function. Adaptin subunits recognise and bind to clathrin through their hinge region (clathrin box), and recruit accessory proteins that modulate AP function through their C-terminal appendage domains. By contrast, GGAs are monomers composed of four domains, which have functions similar to AP subunits: an N-terminal VHS (Vps27p/Hrs/Stam) domain, a GAT (GGA and Tom1) domain, a hinge region, and a C-terminal GAE (gamma-adaptin ear) domain. The GAE domain is similar to the AP gamma-adaptin ear domain, being responsible for the recruitment of accessory proteins that regulate clathrin-mediated endocytosis [].While clathrin mediates endocytic protein transport from ER to Golgi, coatomers (COPI, COPII) primarily mediate intra-Golgi transport, as well as the reverse Golgi to ER transport of dilysine-tagged proteins []. Coatomers reversibly associate with Golgi (non-clathrin-coated) vesicles to mediate protein transport and for budding from Golgi membranes []. Coatomer complexes are hetero-oligomers composed of at least an alpha, beta, beta', gamma, delta, epsilon and zeta subunits. This entry represents a subdomain of the appendage (ear) domain of alpha-adaptin from AP clathrin adaptor complexes, and the appendage domain of the gamma subunit of coatomer complexes. These domains have a three-layer arrangement, alpha-beta-alpha, with a bifurcated antiparallel beta-sheet [, , ]. Although the appendage domains from AP adaptins and coatomers share a similar fold, there is little sequence identity between them. However, they also share similar motif-based cargo recognition and accessory factor recruitment mechanisms. More information about these proteins can be found at Protein of the Month: Clathrin [].

Publication Counts Displayer

1 Child Features

DB identifier Name Short Name Type
IPR003164 Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain Clathrin_a-adaptin_app_sub_C Domain

0 Contains

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
G3DSA:3.30.310.30 GENE3D IPR015873

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

1 Found In

DB identifier Name Short Name Type
IPR017106 Coatomer gamma subunit Coatomer_gsu Family

3 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain   GO:0006886 intracellular protein transport
Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain   GO:0016192 vesicle-mediated transport
Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain   GO:0030117 membrane coat

3 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain   GO:0006886 intracellular protein transport
Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain   GO:0016192 vesicle-mediated transport
Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain   GO:0030117 membrane coat

1 Parent Features

DB identifier Name Short Name Type
IPR009028 Coatomer/calthrin adaptor appendage, C-terminal subdomain Coatomer/calthrin_app_sub_C Domain

32 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0085155 Q8I0G5 Drosophila melanogaster
FBpp0088945 E8NH12 Drosophila melanogaster
FBpp0088490 P91926 Drosophila melanogaster
FBpp0280380 Q29N38 Drosophila pseudoobscura
FBpp0297038 I5ANE0 Drosophila pseudoobscura
FBpp0282417 Q29AE5 Drosophila pseudoobscura
FBpp0167254 B4KFJ2 Drosophila mojavensis
FBpp0172613 B4K7Z2 Drosophila mojavensis
FBpp0234164 B4LTK9 Drosophila virilis
FBpp0237503 B4M106 Drosophila virilis
FBpp0255956 B4PNE7 Drosophila yakuba
FBpp0261149 B4P2D9 Drosophila yakuba
A8XNZ9_CAEBR A8XNZ9 Caenorhabditis briggsae
A8WUZ6_CAEBR A8WUZ6 Caenorhabditis briggsae
K7GXM2_CAEJA K7GXM2 Caenorhabditis japonica
K7I3S0_CAEJA K7I3S0 Caenorhabditis japonica
K7GXM1_CAEJA K7GXM1 Caenorhabditis japonica
H2W9N4_CAEJA H2W9N4 Caenorhabditis japonica
G0NUQ5_CAEBE G0NUQ5 Caenorhabditis brenneri
G0NBB6_CAEBE G0NBB6 Caenorhabditis brenneri
G0MGV3_CAEBE G0MGV3 Caenorhabditis brenneri
G0PIP7_CAEBE G0PIP7 Caenorhabditis brenneri
COPG_CAEEL Q22498 Caenorhabditis elegans
Q2XN02_CAEEL Q2XN02 Caenorhabditis elegans
F5GUA3_CAEEL F5GUA3 Caenorhabditis elegans
Q22601_CAEEL Q22601 Caenorhabditis elegans
AP2A_DROME-2 P91926-2 Drosophila melanogaster
COPG_DROME-2 Q8I0G5-2 Drosophila melanogaster
COPG_DROME-3 Q8I0G5-3 Drosophila melanogaster
G4LU37_DROME G4LU37 Drosophila melanogaster