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Protein Domain : IPR003164

Name  Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain Short Name  Clathrin_a-adaptin_app_sub_C
Type  Domain Description  Proteins synthesized on the ribosome and processed in the endoplasmic reticulum are transported from the Golgi apparatus to the trans-Golgi network (TGN), and from there via small carrier vesicles to their final destination compartment. These vesicles have specific coat proteins (such as clathrin or coatomer) that are important for cargo selection and direction of transport []. Clathrin coats contain both clathrin (acts as a scaffold) and adaptor complexes that link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The two major types of clathrin adaptor complexes are the heterotetrameric adaptor protein (AP) complexes, and the monomeric GGA (Golgi-localising, Gamma-adaptin ear domain homology, ARF-binding proteins) adaptors [, ].AP (adaptor protein) complexes are found in coated vesicles and clathrin-coated pits. AP complexes connect cargo proteins and lipids to clathrin at vesicle budding sites, as well as binding accessory proteins that regulate coat assembly and disassembly (such as AP180, epsins and auxilin). There are different AP complexes in mammals. AP1 is responsible for the transport of lysosomal hydrolases between the TGN and endosomes []. AP2 associates with the plasma membrane and is responsible for endocytosis []. AP3 is responsible for protein trafficking to lysosomes and other related organelles []. AP4 is less well characterised. AP complexes are heterotetramers composed of two large subunits (adaptins), a medium subunit (mu) and a small subunit (sigma). For example, in AP1 these subunits are gamma-1-adaptin, beta-1-adaptin, mu-1 and sigma-1, while in AP2 they are alpha-adaptin, beta-2-adaptin, mu-2 and sigma-2. Each subunit has a specific function. Adaptins recognise and bind to clathrin through their hinge region (clathrin box), and recruit accessory proteins that modulate AP function through their C-terminal ear (appendage) domains. Mu recognises tyrosine-based sorting signals within the cytoplasmic domains of transmembrane cargo proteins []. One function of clathrin and AP2 complex-mediated endocytosis is to regulate the number of GABA(A) receptors available at the cell surface []. AP adaptor alpha-adaptin can be divided into a trunk domain and the C-terminal appendage domain (or ear domain), separated by a linker region. The C-terminal appendage domain regulates translocation of endocytic accessory proteins to the bud site [].This entry represents a subdomain of the appendage (ear) domain of alpha-adaptin from AP clathrin adaptor complexes. This domain has a three-layer arrangement, alpha-beta-alpha, with a bifurcated antiparallel beta-sheet []. More information about these proteins can be found at Protein of the Month: Clathrin [].

Publication Counts Displayer

0 Child Features

0 Contains

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF02296 PFAM IPR003164

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

1 Found In

DB identifier Name Short Name Type
IPR017104 Adaptor protein complex AP-2, alpha subunit AP2_complex_asu Family

3 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain   GO:0006886 intracellular protein transport
Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain   GO:0016192 vesicle-mediated transport
Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain   GO:0030131 clathrin adaptor complex

3 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain   GO:0006886 intracellular protein transport
Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain   GO:0016192 vesicle-mediated transport
Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain   GO:0030131 clathrin adaptor complex

1 Parent Features

DB identifier Name Short Name Type
IPR015873 Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain Clathrin_a/coatomer_app_sub_C Domain

14 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0088945 E8NH12 Drosophila melanogaster
FBpp0088490 P91926 Drosophila melanogaster
FBpp0280380 Q29N38 Drosophila pseudoobscura
FBpp0167254 B4KFJ2 Drosophila mojavensis
FBpp0234164 B4LTK9 Drosophila virilis
FBpp0261149 B4P2D9 Drosophila yakuba
A8XNZ9_CAEBR A8XNZ9 Caenorhabditis briggsae
K7GXM2_CAEJA K7GXM2 Caenorhabditis japonica
K7GXM1_CAEJA K7GXM1 Caenorhabditis japonica
G0NBB6_CAEBE G0NBB6 Caenorhabditis brenneri
G0MGV3_CAEBE G0MGV3 Caenorhabditis brenneri
Q22601_CAEEL Q22601 Caenorhabditis elegans
AP2A_DROME-2 P91926-2 Drosophila melanogaster
E3MAV5_CAERE E3MAV5 Caenorhabditis remanei