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Protein Domain : IPR004030

Name  Nitric oxide synthase, oxygenase domain Short Name  NO_synthase_oxygenase_dom
Type  Domain Description  Nitric oxide synthase () (NOS) enzymes produce nitric oxide (NO) by catalysing a five-electron oxidation of a guanidino nitrogen of L-arginine (L-Arg). Oxidation of L-Arg to L-citrulline occurs via two successive monooxygenation reactions producing N(omega)-hydroxy-L-arginine as an intermediate. 2 mol of O(2) and 1.5 mol of NADPH are consumed per mole of NO formed [].Arginine-derived NO synthesis has been identified in mammals, fish, birds, invertebrates, plants, and bacteria []. Best studied are mammals, where three distinct genes encode NOS isozymes: neuronal (nNOS or NOS-1), cytokine-inducible (iNOS or NOS-2) and endothelial (eNOS or NOS-3) []. iNOS and nNOS are soluble and found predominantly in the cytosol, while eNOS is membrane associated. The enzymes exist as homodimers, each monomer consisting of two major domains: an N-terminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and a C-terminal reductase domain, which is homologous to NADPH:P450 reductase (). The interdomain linker between the oxygenase and reductase domains contains a calmodulin (CaM)-binding sequence. NOSs are the only enzymes known to simultaneously require five bound cofactors animal NOS isozymes are catalytically self-sufficient. The electron flow in the NO synthase reaction is: NADPH -->FAD -->FMN -->haem -->O(2).eNOS localisation to endothelial membranes is mediated by cotranslational N-terminal myristoylation and post-translational palmitoylation []. The subcellular localisation of nNOS in skeletal muscle ismediated by anchoring of nNOS to dystrophin. nNOS contains an additional N-terminal domain, the PDZ domain []. Some bacteria, like Bacillus halodurans, Bacillus subtilisor Deinococcus radiodurans, contain homologues of NOS oxygenase domain. The pattern is directed against the N-terminal haem binding site.This entry represents the oxygenase domain of NOS.

Publication Counts Displayer

0 Child Features

0 Contains

4 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF02898 PFAM IPR004030
PS60001 PROSITE IPR004030
G3DSA:3.90.340.10 GENE3D IPR004030
SSF56512 SSF IPR004030

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

2 Found In

DB identifier Name Short Name Type
IPR012144 Nitric-oxide synthase, eukaryote NOS_euk Family
IPR017142 Nitric oxide synthase, oxygenase subunit Nitric_oxide_synthase_Oase-su Family

3 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Nitric oxide synthase, oxygenase domain   GO:0004517 nitric-oxide synthase activity
Nitric oxide synthase, oxygenase domain   GO:0006809 nitric oxide biosynthetic process
Nitric oxide synthase, oxygenase domain   GO:0055114 oxidation-reduction process

3 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Nitric oxide synthase, oxygenase domain   GO:0004517 nitric-oxide synthase activity
Nitric oxide synthase, oxygenase domain   GO:0006809 nitric oxide biosynthetic process
Nitric oxide synthase, oxygenase domain   GO:0055114 oxidation-reduction process

0 Parent Features

12 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0079777 Q27571 Drosophila melanogaster
FBpp0287477 Q29KP6 Drosophila pseudoobscura
FBpp0167029 B4KKM7 Drosophila mojavensis
FBpp0232056 B4LS74 Drosophila virilis
FBpp0258305 B4P121 Drosophila yakuba
B6IKK8_CAEBR B6IKK8 Caenorhabditis briggsae
NOS_DROME-6 Q27571-6 Drosophila melanogaster
NOS_DROME-2 Q27571-2 Drosophila melanogaster
NOS_DROME-3 Q27571-3 Drosophila melanogaster
NOS_DROME-4 Q27571-4 Drosophila melanogaster
NOS_DROME-7 Q27571-7 Drosophila melanogaster
NOS_DROME-5 Q27571-5 Drosophila melanogaster