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Protein Domain : IPR002553

Name  Clathrin/coatomer adaptor, adaptin-like, N-terminal Short Name  Clathrin/coatomer_adapt-like_N
Type  Domain Description  Proteins synthesized on the ribosome and processedin the endoplasmic reticulum are transported from the Golgi apparatus to the trans-Golgi network (TGN), and from there via small carrier vesicles to their final destination compartment. This traffic is bidirectional, to ensure that proteins required to form vesicles are recycled. Vesicles have specific coat proteins (such as clathrin or coatomer) that are important for cargo selection and direction of transfer []. Clathrin coats contain both clathrin and adaptor complexes that link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The two major types of clathrin adaptor complexes are the heterotetrameric adaptor protein (AP) complexes, and the monomeric GGA (Golgi-localising, Gamma-adaptin ear domain homology, ARF-binding proteins) adaptors []. All AP complexes are heterotetramers composed of two large subunits (adaptins), a medium subunit (mu) and a small subunit (sigma). Each subunit has a specific function. Adaptin subunits recognise and bind to clathrin through their hinge region (clathrin box), and recruit accessory proteins that modulate AP function through their C-terminal appendage domains. By contrast, GGAs are monomers composed of four domains, which have functions similar to AP subunits: an N-terminal VHS (Vps27p/Hrs/Stam) domain, a GAT (GGA and Tom1) domain, a hinge region, and a C-terminal GAE (gamma-adaptin ear) domain. The GAE domain is similar to the AP gamma-adaptin ear domain, being responsible for the recruitment of accessory proteins that regulate clathrin-mediated endocytosis [].While clathrin mediates endocytic protein transport from ER to Golgi, coatomers (COPI, COPII) primarily mediate intra-Golgi transport, as well as the reverse Golgi to ER transport of dilysine-tagged proteins []. Coatomers reversibly associate with Golgi (non-clathrin-coated) vesicles to mediate protein transport and for budding from Golgi membranes []. Coatomer complexes are hetero-oligomers composed of at least an alpha, beta, beta', gamma, delta, epsilon and zeta subunits. This entry represents the N-terminal domain of various adaptins from different AP clathrin adaptor complexes (including AP1, AP2, AP3 and AP4), and from the beta and gamma subunits of various coatomer (COP) adaptors. This domain has a 2-layer alpha/alpha fold that forms a right-handed superhelix, and is a member of the ARM repeat superfamily []. The N-terminal region of the various AP adaptor proteins share strong sequence identity; by contrast, the C-terminal domains of different adaptins share similar structural folds, but have little sequence identity []. It has been proposed that the N-terminal domain interacts with another uniform component of the coated vesicles.

Publication Counts Displayer

0 Child Features

1 Contains

DB identifier Name Short Name Type
IPR011989 Armadillo-like helical ARM-like Domain

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF01602 PFAM IPR002553

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

7 Found In

DB identifier Name Short Name Type
IPR017107 Adaptor protein complex AP-1, gamma subunit AP1_complex_gsu Family
IPR017105 Adaptor protein complex AP-3, delta subunit AP3_complex_dsu Family
IPR016460 Coatomer beta subunit (COPB1) COPB1 Family
IPR017104 Adaptor protein complex AP-2, alpha subunit AP2_complex_asu Family
IPR017106 Coatomer gamma subunit Coatomer_gsu Family
IPR016342 AP-1, 2,4 complex subunit beta AP_complex_bsu_1_2_4 Family
IPR017109 Adaptor protein complex AP-4, epsilon subunit AP4_complex_esu Family

3 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Clathrin/coatomer adaptor, adaptin-like, N-terminal   GO:0006886 intracellular protein transport
Clathrin/coatomer adaptor, adaptin-like, N-terminal   GO:0016192 vesicle-mediated transport
Clathrin/coatomer adaptor, adaptin-like, N-terminal   GO:0030117 membrane coat

3 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Clathrin/coatomer adaptor, adaptin-like, N-terminal   GO:0006886 intracellular protein transport
Clathrin/coatomer adaptor, adaptin-like, N-terminal   GO:0016192 vesicle-mediated transport
Clathrin/coatomer adaptor, adaptin-like, N-terminal   GO:0030117 membrane coat

0 Parent Features

110 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0085155 Q8I0G5 Drosophila melanogaster
FBpp0074558 Q24253 Drosophila melanogaster
FBpp0088945 E8NH12 Drosophila melanogaster
FBpp0088490 P91926 Drosophila melanogaster
FBpp0301194 Q86B59 Drosophila melanogaster
FBpp0304098 M9PHJ5 Drosophila melanogaster
FBpp0304099 M9PJM7 Drosophila melanogaster
FBpp0071231 Q9W388 Drosophila melanogaster
FBpp0071230 Q7KVR7 Drosophila melanogaster
FBpp0071232 Q7KVR8 Drosophila melanogaster
FBpp0070670 Q9W4K1 Drosophila melanogaster
FBpp0074348 P45437 Drosophila melanogaster
FBpp0272566 Q29H04 Drosophila pseudoobscura
FBpp0272222 Q29G21 Drosophila pseudoobscura
FBpp0273370 Q29J51 Drosophila pseudoobscura
FBpp0280380 Q29N38 Drosophila pseudoobscura
FBpp0280960 B5DN54 Drosophila pseudoobscura
FBpp0297038 I5ANE0 Drosophila pseudoobscura
FBpp0282417 Q29AE5 Drosophila pseudoobscura
FBpp0284323 Q29HV6 Drosophila pseudoobscura
FBpp0163656 B4L8M3 Drosophila mojavensis
FBpp0164750 B4L3F0 Drosophila mojavensis
FBpp0164816 B4L3P9 Drosophila mojavensis
FBpp0165315 B4L6V2 Drosophila mojavensis
FBpp0167254 B4KFJ2 Drosophila mojavensis
FBpp0170686 B4L5S4 Drosophila mojavensis
FBpp0170977 B4L5M8 Drosophila mojavensis
FBpp0172613 B4K7Z2 Drosophila mojavensis
FBpp0229295 B4MET3 Drosophila virilis
FBpp0229619 B4MD51 Drosophila virilis