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Protein Domain : IPR013078

Name  Histidine phosphatase superfamily, clade-1 Short Name  His_Pase_superF_clade-1
Type  Family Description  The histidine phosphatase superfamily is so named because catalysiscentres on a conserved His residue that is transiently phosphorylatedduring the catalytic cycle. Other conserved residues contribute to a'phosphate pocket' and interact with the phospho group of substratebefore, during and after its transfer to the His residue. Structure andsequence analyses show that different families contribute differentadditional residues to the 'phosphate pocket' and, more surprisingly,differ in the position, in sequence and in three dimensions, of acatalytically essential acidic residue. The superfamily may be dividedinto two main branches. The relationship between the two branches isnot evident by (PSI-)BLAST but is clear from more sensitive sequencesearches and structural comparisons [].The larger clade-1 contains a wide variety of catalytic functions, thebest known being fructose 2,6-bisphosphatase (found in a bifunctionalprotein with 2-phosphofructokinase) and cofactor-dependentphosphoglycerate mutase. The latter is an unusual example of a mutaseactivity in the superfamily: the vast majority of members appear to bephosphatases. The bacterial regulatory protein phosphatase SixA is alsoin clade-1 and has a minimal, and possible ancestral-like structure,lacking the large domain insertions that contribute to binding of smallmolecules in clade-1 members.Phosphoglycerate mutase () (PGAM) and bisphosphoglycerate mutase () (BPGM) are structurally related enzymes that catalyse reactions involving the transfer of phospho groups between the three carbon atoms of phosphoglycerate [, , ]. Both enzymes can catalyse three different reactions with different specificities, the isomerization of 2-phosphoglycerate (2-PGA) to 3-phosphoglycerate (3-PGA) with 2,3-diphosphoglycerate (2,3-DPG) as the primer of the reaction, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA (phosphatase activity).In mammals, PGAM is a dimeric protein with two isoforms, the M (muscle) and B (brain) forms. In yeast, PGAM is a tetrameric protein.BPGM is a dimeric protein and is found mainly in erythrocytes where it plays a major role in regulating haemoglobin oxygen affinity as a consequence of controlling 2,3-DPG concentration. The catalytic mechanism of both PGAM and BPGM involves the formation of a phosphohistidine intermediate [].A number of other proteins including, the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase []that catalyses both the synthesis and the degradation of fructose-2,6-bisphosphate and bacterial alpha-ribazole-5'-phosphate phosphatase, which is involved in cobalamin biosynthesis, contain this domain [].

Publication Counts Displayer

12 Child Features

DB identifier Name Short Name Type
IPR003094 Fructose-2,6-bisphosphatase 6Pfruct_kin Family
IPR005952 Phosphoglycerate mutase 1 Phosphogly_mut1 Family
IPR015845 Agropine synthesis reductase Mas1 Family
IPR011310 Lipopolysaccharide core heptose(II)-phosphate phosphatase LipoPS_heptP_Pase Family
IPR017578 Alpha-ribazole phosphatase, CobC Ribazole_CobC Family
IPR017070 Uncharacterised conserved protein UCP036920, phosphoglycerate mutase, plant X4/Y4 UCP036920_PGAM-like_plant Family
IPR023086 Phosphoglycerate mutase, GpmB, probable Phosphoglycerate_mutase_GpmB Family
IPR014623 Transcription factor TFIIIC, tau55 subunit Tau55_TFC7 Family
IPR014636 RNase H, phosphoglycerate mutase domain-containing RNaseH/PGlycerate_mutase Family
IPR022492 Phosphomutase MSMEG4193, putative Phosphomutase_MSMEG4193_put Family
IPR004449 Phosphohistidine phosphatase SixA, subgroup SixA_subgr Family
IPR012398 PRIB5 PRIB5 Family

0 Contains

2 Cross References

Source . Name

Subject . Primary Identifier
PF00300 PFAM IPR013078
SM00855 SMART IPR013078

2 Data Sets

Name URL
Swiss-Prot data set
InterPro data set  

0 Found In

0 GO Annotation

0 Ontology Annotations

1 Parent Features

DB identifier Name Short Name Type
IPR029033 Histidine phosphatase superfamily His_PPase_superfam Family

100 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0074487 Q0KHQ6 Drosophila melanogaster
FBpp0074486 Q9VWH7 Drosophila melanogaster
FBpp0099919 Q9VAN7 Drosophila melanogaster
FBpp0072263 Q9W173 Drosophila melanogaster
FBpp0112189 A8JR97 Drosophila melanogaster
FBpp0083896 Q9VCE9 Drosophila melanogaster
FBpp0112188 Q86PB2 Drosophila melanogaster
FBpp0083616 Q8T8W6 Drosophila melanogaster
FBpp0083617 Q86BN5 Drosophila melanogaster
FBpp0083618 Q86BN6 Drosophila melanogaster
FBpp0082089 Q9VGB6 Drosophila melanogaster
FBpp0070350 O46084 Drosophila melanogaster
FBpp0282012 Q298M0 Drosophila pseudoobscura
FBpp0300366 I5ANX8 Drosophila pseudoobscura
FBpp0283608 Q29AB2 Drosophila pseudoobscura
FBpp0282923 Q29HG0 Drosophila pseudoobscura
FBpp0272859 Q29IW2 Drosophila pseudoobscura
FBpp0278733 Q28WV5 Drosophila pseudoobscura
FBpp0281457 Q295R2 Drosophila pseudoobscura
FBpp0282058 Q298T2 Drosophila pseudoobscura
FBpp0300253 I5ANV0 Drosophila pseudoobscura
FBpp0159497 B4KDY1 Drosophila mojavensis
FBpp0165308 B4L6W6 Drosophila mojavensis
FBpp0165637 B4L6S9 Drosophila mojavensis
FBpp0168123 B4KU84 Drosophila mojavensis
FBpp0171598 B4K5D6 Drosophila mojavensis
FBpp0172409 B4KAZ1 Drosophila mojavensis
FBpp0172672 B4K6V1 Drosophila mojavensis
FBpp0225504 B4M5B8 Drosophila virilis
FBpp0228925 B4MBT4 Drosophila virilis