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Protein Domain : IPR010114

Name  Signal transduction response regulator, nitrogen regulation NR(I) Short Name  Sig_transdc_resp-reg_NtrC
Type  Family Description  Two-component signal transduction systems enable bacteria to sense, respond, and adapt to a wide range of environments, stressors, and growth conditions []. Some bacteria can contain up to as many as 200 two-component systems that need tight regulation to prevent unwanted cross-talk []. These pathways have been adapted to response to a wide variety of stimuli, including nutrients, cellular redox state, changes in osmolarity, quorum signals, antibiotics, and more []. Two-component systems are comprised of a sensor histidine kinase (HK) and its cognate response regulator (RR) []. The HK catalyses its own auto-phosphorylation followed by the transfer of the phosphoryl group to the receiver domain on RR; phosphorylation of the RR usually activates an attached output domain, which can then effect changes in cellular physiology, often by regulating gene expression. Some HK are bifunctional, catalysing both the phosphorylation and dephosphorylation of their cognate RR. The input stimuli can regulate either the kinase or phosphatase activity of the bifunctional HK.A variant of the two-component system is the phospho-relay system. Here a hybrid HK auto-phosphorylates and then transfers the phosphoryl group to an internal receiver domain, rather than to a separate RR protein. The phosphoryl group is then shuttled to histidine phosphotransferase (HPT) and subsequently to a terminal RR, which can evoke the desired response [, ].This entry represents Nitrogen regulatory protein C (NtrC), which is a bacterial enhancer-binding protein that activates the transcription of genes encoding enzymes required for nitrogen metabolism. It is phosphorylated by NtrB and interacts with sigma-54. One of the best studied examples is its activation of the gene glnA, which encodes the enzyme glutamine synthetase [].NtrC is composed of three domains [, ]. The 124 residue N-terminal domain is homologous to receiver domains of other response regulator proteins in two-component signal transduction systems [, ]. The 240 residue central domain of NtrC is homologous to a domain found in all activators of the sigma-54 RNA polymerase holoenzyme [, ]. The C-terminal domain has been indicated to contain the determinants necessary for both DNA-binding and dimerization of full-length NtrC.

Publication Counts Displayer

0 Child Features

4 Contains

DB identifier Name Short Name Type
IPR009057 Homeodomain-like Homeodomain-like Domain
IPR001789 Signal transduction response regulator, receiver domain Sig_transdc_resp-reg_receiver Domain
IPR002078 RNA polymerase sigma factor 54 interaction domain Sigma_54_int Domain
IPR002197 DNA binding HTH domain, Fis-type HTH_Fis Domain

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
TIGR01818 TIGRFAMs IPR010114

2 Data Sets

Name URL
InterPro data set  
InterPro GO Annotation data set  

0 Found In

5 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Signal transduction response regulator, nitrogen regulation NR(I)   GO:0000156 phosphorelay response regulator activity
Signal transduction response regulator, nitrogen regulation NR(I)   GO:0003677 DNA binding
Signal transduction response regulator, nitrogen regulation NR(I)   GO:0005524 ATP binding
Signal transduction response regulator, nitrogen regulation NR(I)   GO:0000160 phosphorelay signal transduction system
Signal transduction response regulator, nitrogen regulation NR(I)   GO:0006808 regulation of nitrogen utilization

5 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Signal transduction response regulator, nitrogen regulation NR(I)   GO:0000156 phosphorelay response regulator activity
Signal transduction response regulator, nitrogen regulation NR(I)   GO:0003677 DNA binding
Signal transduction response regulator, nitrogen regulation NR(I)   GO:0005524 ATP binding
Signal transduction response regulator, nitrogen regulation NR(I)   GO:0000160 phosphorelay signal transduction system
Signal transduction response regulator, nitrogen regulation NR(I)   GO:0006808 regulation of nitrogen utilization

0 Parent Features

0 Proteins