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Protein Domain : IPR018109

Name  Folylpolyglutamate synthetase, conserved site Short Name  Folylpolyglutamate_synth_CS
Type  Conserved_site Description  Folylpolyglutamate synthase (FPGS) is an ATP-dependent enzyme that is responsible for the addition of a polyglutamate tail to folate and folate derivatives []. It plays a key role in the retention of the intracellular folate pool. The crystal structure of the MgATP complex of the enzyme from Lactobacillus casei reveals FPGS to be a modular protein, consisting of two domains, one with a typical mononucleotide-binding fold and the other similar to the folate-binding enzyme dihydrofolate reductase. The active site of the enzyme is located in a large interdomain cleft adjacent to an ATP-binding P-loop motif. Opposite this site, in the C domain, a cavity likely to be the folate binding site has been identified, and inspection of this cavity and the surrounding protein structure suggests that the glutamate tail of the substrate may project into the active site. A further feature of the structure is a well defined Omega loop, which contributes both to the active site and to interdomain interactions [].This entry represents a conserved site found in the FPGS enzymes.

Publication Counts Displayer

0 Child Features

0 Contains

2 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PS01011 PROSITE IPR018109
PS01012 PROSITE IPR018109

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

2 Found In

DB identifier Name Short Name Type
IPR001645 Folylpolyglutamate synthetase Folylpolyglutamate_synth Family
IPR013221 Mur ligase, central Mur_ligase_cen Domain

3 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Folylpolyglutamate synthetase, conserved site   GO:0004326 tetrahydrofolylpolyglutamate synthase activity
Folylpolyglutamate synthetase, conserved site   GO:0005524 ATP binding
Folylpolyglutamate synthetase, conserved site   GO:0009396 folic acid-containing compound biosynthetic process

3 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Folylpolyglutamate synthetase, conserved site   GO:0004326 tetrahydrofolylpolyglutamate synthase activity
Folylpolyglutamate synthetase, conserved site   GO:0005524 ATP binding
Folylpolyglutamate synthetase, conserved site   GO:0009396 folic acid-containing compound biosynthetic process

0 Parent Features

13 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0073488 Q9VYL1 Drosophila melanogaster
FBpp0273355 Q29J67 Drosophila pseudoobscura
FBpp0165602 B4L6K6 Drosophila mojavensis
FBpp0231177 B4M880 Drosophila virilis
FBpp0262028 B4Q1P0 Drosophila yakuba
A8X7Y9_CAEBR A8X7Y9 Caenorhabditis briggsae
H2WNP9_CAEJA H2WNP9 Caenorhabditis japonica
G0MLK2_CAEBE G0MLK2 Caenorhabditis brenneri
FOLC_CAEEL Q09509 Caenorhabditis elegans
FOLC_CAEEL-3 Q09509-3 Caenorhabditis elegans
FOLC_CAEEL-2 Q09509-2 Caenorhabditis elegans
Q8MRU3_DROME Q8MRU3 Drosophila melanogaster
E3LYQ3_CAERE E3LYQ3 Caenorhabditis remanei