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Protein Domain : IPR003109

Name  GoLoco motif Short Name  GoLoco_motif
Type  Conserved_site Description  In heterotrimeric G-protein signalling, cell surface receptors (GPCRs) arecoupled to membrane-associated heterotrimers comprising a GTP-hydrolysing subunit G-alpha and a G-beta/G-gamma dimer. The inactive form contains the alpha subunit bound to GDP and complexes with the beta and gamma subunit. When the ligand is associated to the receptor, GDP is displaced from G-alpha and GTP is bound. GTP/G-alpha complex dissociates from the trimer and associates to an effector until the intrinsic GTPase activity of G-alpha returns the protein to GDP bound form. Reassociation of GDP bound G-alpha with G-beta/G-gamma dimer terminates the signal. Several mechanisms regulate the signal output at different stage of the G-protein cascade. Two classes of intracellular proteins act as inhibitors of G protein activation: GTPase activating proteins (GAPs), which enhance GTP hydrolysis (see ),and guanine dissociation inhibitors (GDIs), which inhibit GDP dissociation.The GoLoco or G-protein regulatory (GPR) motif found in various G-proteinregulators [, ]acts as a GDI on G-alpha(i) [, ].The crystal structure of the GoLoco motif in complex with G-alpha(i) has been solved []. It consists of three small alpha helices. The highly conserved Asp-Gln-Arg triad within the GoLoco motif participates directly in GDP binding by extending the arginine side chain into the nucleotide binding pocket, highly reminiscent of the catalytic arginine finger employed in GTPase-activating protein (see ). This addition of an arginine in the binding pocket affects the interaction of GDP with G-alpha and therefore is certainly important for the GoLoco GDI activity [].Some proteins known to contain a GoLoco motif are listed below:Mammalian regulators of G-protein signalling 12 and 14 (RGS12 and RGS14), multifaceted signal transduction regulators.Loco, the drosophila RGS12 homologue.Mammalian Purkinje-cell protein-2 (Pcp2). It may function as a cell-type specific modulator for G protein-mediated cell signalling. It is uniquely expressed in cerebellar Purkinje cells and in retinal bipolar neurons.Eukaryotic Rap1GAP. A GTPase activator for the nuclear ras-related regulatory protein RAP-1A.Drosophila protein Rapsynoid (also known as Partner of Inscuteable, Pins) and its mammalian homologues AGS3 and LGN. They form a G-protein regulator family that also contains TPR repeats.

Publication Counts Displayer

0 Child Features

0 Contains

3 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF02188 PFAM IPR003109
PS50877 PROFILE IPR003109
SM00390 SMART IPR003109

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

0 Found In

1 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
GoLoco motif   GO:0030695 GTPase regulator activity

1 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
GoLoco motif   GO:0030695 GTPase regulator activity

0 Parent Features

49 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0084559 Q9VB22 Drosophila melanogaster
FBpp0083669 Q8IN00 Drosophila melanogaster
FBpp0083668 Q9VCX2 Drosophila melanogaster
FBpp0083666 Q9VCX1 Drosophila melanogaster
FBpp0306873 A8DYW8 Drosophila melanogaster
FBpp0306872 Q59DZ5 Drosophila melanogaster
FBpp0306870 Q9VM00 Drosophila melanogaster
FBpp0301188 D5A7N8 Drosophila melanogaster
FBpp0293919 I5AN25 Drosophila pseudoobscura
FBpp0293918 B5DYK9 Drosophila pseudoobscura
FBpp0293920 I5AN26 Drosophila pseudoobscura
FBpp0293917 I5AN24 Drosophila pseudoobscura
FBpp0280180 B5DHY9 Drosophila pseudoobscura
FBpp0283297 Q29BH6 Drosophila pseudoobscura
FBpp0159736 B4K7N8 Drosophila mojavensis
FBpp0171228 B4KBA4 Drosophila mojavensis
FBpp0171229 B4KBA3 Drosophila mojavensis
FBpp0172305 B4KL26 Drosophila mojavensis
FBpp0224735 B4M420 Drosophila virilis
FBpp0224880 B4M633 Drosophila virilis
FBpp0238027 B4LUQ9 Drosophila virilis
FBpp0263361 B4NZJ5 Drosophila yakuba
FBpp0268756 B4PRC7 Drosophila yakuba
FBpp0269030 B4PMC9 Drosophila yakuba
A8XKD8_CAEBR A8XKD8 Caenorhabditis briggsae
A8XAE1_CAEBR A8XAE1 Caenorhabditis briggsae
A8X3P6_CAEBR A8X3P6 Caenorhabditis briggsae
H2W074_CAEJA H2W074 Caenorhabditis japonica
K7HCR3_CAEJA K7HCR3 Caenorhabditis japonica
K7HCR2_CAEJA K7HCR2 Caenorhabditis japonica