modENCODE |  Help |  blog

Protein Domain : IPR003694

Name  NAD(+) synthetase Short Name  NAD_synthase
Type  Family Description  This entry represents NAD(+) synthetases, including glutamine-dependent NAD(+) synthetases and NH(3)-dependent NAD(+) synthetases.NAD+ is involved electron transport and redox reactions and in DNA ligation and protein ADP-ribosylation. In yeast and most other organisms, NAD is generated through the de novo pathway and the salvage pathway. In the de novo pathway, quinolinic acid is converted to nicotinic acid mononucleotide (NaMN). In the salvage pathway, NaMN is generated by recycling of nicotinamide. Both pathways converge on NaMN, which is then converted into deamido-NAD+. Subsequently, deamido-NAD+ is converted to NAD+ by NAD+ synthetase [].NAD+ synthetase has been extensively studied in bacteria. It is encoded by nadE gene in E. coli and by outB gene in B. subtilis []. These prokaryotic enzymes are ammonia-dependent (containing an ammonia-utilising domain)[]. However, some pokaryotic NAD(+) synthetases, such as that from Mycobacterium tuberculosis, contain a nitrilase-related domain, are glutamine-dependent []. Eukaryotic NAD+ synthetases are glutamine-dependent. In budding yeast, this enzyme is named as Qns1. It contains an N-terminal nitrilase-related domain (contaiing the nitrilase-related active-site residues) and a C-terminal NAD+ synthetase domain []. Both domains are required for its function in vivo. The nitrilase-related domain is the fourth independently evolved glutamine amidotransferase domain to have been identified in nature [].

Publication Counts Displayer

2 Child Features

DB identifier Name Short Name Type
IPR014445 Glutamine-dependent NAD(+) synthetase Gln-dep_NAD_synthase Family
IPR022926 NH(3)-dependent NAD(+) synthetase NH(3)-dep_NAD(+)_synth Family

0 Contains

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
TIGR00552 TIGRFAMs IPR003694

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

0 Found In

3 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
NAD(+) synthetase   GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity
NAD(+) synthetase   GO:0005524 ATP binding
NAD(+) synthetase   GO:0009435 NAD biosynthetic process

3 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
NAD(+) synthetase   GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity
NAD(+) synthetase   GO:0005524 ATP binding
NAD(+) synthetase   GO:0009435 NAD biosynthetic process

0 Parent Features

13 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0073676 Q9VYA0 Drosophila melanogaster
FBpp0284344 Q29HW0 Drosophila pseudoobscura
FBpp0163360 B4L7J5 Drosophila mojavensis
FBpp0233494 B4M2T6 Drosophila virilis
FBpp0261155 B4Q2E8 Drosophila yakuba
A8WUY2_CAEBR A8WUY2 Caenorhabditis briggsae
H2VTT6_CAEJA H2VTT6 Caenorhabditis japonica
G0NUR4_CAEBE G0NUR4 Caenorhabditis brenneri
G0PK32_CAEBE G0PK32 Caenorhabditis brenneri
F3Y5P7_CAEEL F3Y5P7 Caenorhabditis elegans
Q9XXK6_CAEEL Q9XXK6 Caenorhabditis elegans
F3Y5P6_CAEEL F3Y5P6 Caenorhabditis elegans
E3MM46_CAERE E3MM46 Caenorhabditis remanei