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Protein Domain : IPR016260

Name  Bifunctional 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphate 2-phosphatase Short Name  Bifunct_6PFK/fruc_bisP_Ptase
Type  Family Description  Fructose 2,6-bisphosphate is a signal molecule important for control of glycolysis. Its formation from fructose 6-phosphate and Mg-ATP is catalysed by 6-phosphofructo-2-kinase (), while its degradation is catalysed by fructose-2,6-bisphosphatase (). In plants and animals these reactions are effected by a bifunctional, two-domain enzyme. Some of the related proteins found in fungi and trypanosomatids have lost one or the other activity--though still possessing the two-domain structure--indicating that the gene fusion that produced the bifunctional molecule occurred early in eukaryote evolution []. Yeast 6-phosphofructo-2-kinase Pfk26p, for example, has about 42% identity with the bifunctional enzyme from rat liver, with extra sequence at each end. Although the sequences are very similar in the bisphosphatase domain, the essential histidine of the liver enzyme is replaced by a serine in yeast, explaining the apparent lack of bisphosphatase activity. Also, the yeast enzyme, which is activated by protein kinase A, has a putative phosphorylation site near its carboxyl end and lacks the N-terminal phosphorylation sequence involved in inhibition of the liver enzyme [].In animals, yeast, and trypanosomatids, several isozymes (and sometimes several isoforms of each) withdifferent kinetic and regulatory mechanisms are found, allowing for adaptation to various hormonal, nutritional, and environmental states. The major mammalian isoenzymes are characteristic of liver, heart, brain/placenta, and testis. In contrast, only a single gene has been found in plants [].The related represents a small group of fungal proteins from which the fructose-2,6-bisphosphatase domain has been lost.

Publication Counts Displayer

0 Child Features

1 Contains

DB identifier Name Short Name Type
IPR013079 6-phosphofructo-2-kinase 6Phosfructo_kin Domain

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PIRSF000709 PIRSF IPR016260

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

0 Found In

2 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Bifunctional 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphate 2-phosphatase   GO:0004331 fructose-2,6-bisphosphate 2-phosphatase activity
Bifunctional 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphate 2-phosphatase   GO:0006003 fructose 2,6-bisphosphate metabolic process

2 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Bifunctional 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphate 2-phosphatase   GO:0004331 fructose-2,6-bisphosphate 2-phosphatase activity
Bifunctional 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphate 2-phosphatase   GO:0006003 fructose 2,6-bisphosphate metabolic process

1 Parent Features

DB identifier Name Short Name Type
IPR003094 Fructose-2,6-bisphosphatase 6Pfruct_kin Family

20 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0074487 Q0KHQ6 Drosophila melanogaster
FBpp0074486 Q9VWH7 Drosophila melanogaster
FBpp0272859 Q29IW2 Drosophila pseudoobscura
FBpp0165308 B4L6W6 Drosophila mojavensis
FBpp0230157 B4MA41 Drosophila virilis
FBpp0260856 B4PZH7 Drosophila yakuba
A8XBW5_CAEBR A8XBW5 Caenorhabditis briggsae
A8X920_CAEBR A8X920 Caenorhabditis briggsae
H2W661_CAEJA H2W661 Caenorhabditis japonica
H2VI25_CAEJA H2VI25 Caenorhabditis japonica
G0NVZ7_CAEBE G0NVZ7 Caenorhabditis brenneri
G0P8K7_CAEBE G0P8K7 Caenorhabditis brenneri
G0MF57_CAEBE G0MF57 Caenorhabditis brenneri
F26_CAEEL Q21122 Caenorhabditis elegans
Q9N590_CAEEL Q9N590 Caenorhabditis elegans
Q95Y85_CAEEL Q95Y85 Caenorhabditis elegans
Q9Y1W3_DROME Q9Y1W3 Drosophila melanogaster
C3KGL9_DROME C3KGL9 Drosophila melanogaster
E3NKD2_CAERE E3NKD2 Caenorhabditis remanei
E3MSF0_CAERE E3MSF0 Caenorhabditis remanei