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Protein Domain : IPR019804

Name  Ras guanine-nucleotide exchange factor, conserved site Short Name  Ras_G-nucl-exch_fac_CS
Type  Conserved_site Description  Ras proteins are membrane-associated molecular switches that bind GTP and GDP and slowly hydrolyse GTP to GDP []. The balance between the GTP bound (active) and GDP bound (inactive) states is regulated by the opposite action of proteins activating the GTPase activity and that of proteins which promote the loss of bound GDP and the uptake of fresh GTP [, ]. The latter proteins are known as guanine-nucleotide dissociation stimulators (GDSs) (or also as guanine-nucleotide releasing (or exchange) factors (GRFs)). Proteins that act as GDS can be classified into at least two families, on the basis of sequence similarities, the CDC24 family (see ) and the CDC25 family.The size of the proteins of the CDC25 family range from 309 residues (LTE1) to 1596 residues (sos). The sequence similarity shared by all these proteins is limited to a region of about 250 amino acids generally located in their C-terminal section (currently the only exceptions are sos and ralGDS where this domain makes up the central part of the protein). This domain has been shown, in CDC25 an SCD25, to be essential for the activity of these proteins.The crystal structure of the GEF region of human Sos1 complexes with Ras has been solved []. The structure consists of two distinct alpha helical structural domains: the N-terminal domain which seems to have a purely structural role and the C-terminal domain which is sufficient for catalytic activity and contains all residues that interact with Ras. A main feature of the catalytic domain is the protrusion of a helical hairpin important for the nucleotide-exchange mechanism. The N-terminal domain is likely to be important for the stability and correct placement of the hairpin structure. The signature pattern for this entry spans the helical hairpin.

Publication Counts Displayer

0 Child Features

0 Contains

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PS00720 PROSITE IPR019804

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

2 Found In

DB identifier Name Short Name Type
IPR001895 Guanine-nucleotide dissociation stimulator CDC25 RasGRF_CDC25 Domain
IPR015758 Ral guanine nucleotide dissociation stimulator RalGDS Family

2 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Ras guanine-nucleotide exchange factor, conserved site   GO:0005085 guanyl-nucleotide exchange factor activity
Ras guanine-nucleotide exchange factor, conserved site   GO:0007264 small GTPase mediated signal transduction

2 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Ras guanine-nucleotide exchange factor, conserved site   GO:0005085 guanyl-nucleotide exchange factor activity
Ras guanine-nucleotide exchange factor, conserved site   GO:0007264 small GTPase mediated signal transduction

0 Parent Features

85 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0289316 B7Z026 Drosophila melanogaster
FBpp0289315 B7Z025 Drosophila melanogaster
FBpp0078879 Q9VMF3 Drosophila melanogaster
FBpp0080118 P26675 Drosophila melanogaster
FBpp0086095 A1ZAU6 Drosophila melanogaster
FBpp0075535 Q9U4U5 Drosophila melanogaster
FBpp0075536 Q86BI2 Drosophila melanogaster
FBpp0078182 Q7KTU3 Drosophila melanogaster
FBpp0289070 B7Z132 Drosophila melanogaster
FBpp0289073 B7Z131 Drosophila melanogaster
FBpp0289753 O77086 Drosophila melanogaster
FBpp0289072 B7Z130 Drosophila melanogaster
FBpp0111548 Q7K3Z6 Drosophila melanogaster
FBpp0289071 B7Z129 Drosophila melanogaster
FBpp0303219 M9PCE9 Drosophila melanogaster
FBpp0306980 M9PJ95 Drosophila melanogaster
FBpp0306982 M9PGJ9 Drosophila melanogaster
FBpp0274406 Q2M017 Drosophila pseudoobscura
FBpp0274612 B5DQP9 Drosophila pseudoobscura
FBpp0272752 Q29IL8 Drosophila pseudoobscura
FBpp0277143 B5E1B7 Drosophila pseudoobscura
FBpp0277632 Q291F9 Drosophila pseudoobscura
FBpp0285623 Q29JN4 Drosophila pseudoobscura
FBpp0287317 B5DK13 Drosophila pseudoobscura
FBpp0160737 B4KFY7 Drosophila mojavensis
FBpp0162642 B4KYJ5 Drosophila mojavensis
FBpp0162673 B4KYV6 Drosophila mojavensis
FBpp0167457 B4KIX8 Drosophila mojavensis
FBpp0167805 B4KQX8 Drosophila mojavensis
FBpp0169448 B4KNH3 Drosophila mojavensis