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Protein Domain : IPR000219

Name  Dbl homology (DH) domain Short Name  DH-domain
Type  Domain Description  The Rho family GTPases Rho, Rac and CDC42 regulate a diverse array of cellular processes. Like all members of the Ras superfamily, the Rho proteins cycle between active GTP-bound and inactive GDP-bound conformational states.Activation of Rho proteins through release of bound GDP and subsequentbinding of GTP, is catalysed by guanine nucleotide exchange factors (GEFs) inthe Dbl family. The proteins encoded by members of the Dbl family share acommon domain, presented in this entry, of about 200 residues (designated the Dbl homology or DH domain) that has been shown to encode a GEF activity specific for a number of Rho family members. In addition, all family members possess a second, shared domain designated the pleckstrin homology (PH) domain (). Trio and its homologue UNC-73 are unique within the Dbl family insomuch as they encode two distinct DH/PH domain modules. The PH domain is invariably located immediately C-terminal to the DH domain and this invariant topography suggests a functional interdependence between these two structural modules. Biochemical data have established the role of the conserved DH domain in Rho GTPase interaction and activation, and the role of the tandem PH domain in intracellular targeting and/or regulation of DH domain function. The DH domain of Dbl has been shown to mediate oligomerisation that is mostly homophilic in nature. In addition to the tandem DH/PH domains Dbl family GEFs contain diverse structural motifs like serine/threonine kinase, RBD, PDZ, RGS, IQ, REM, Cdc25, RasGEF, CH, SH2, SH3, EF, spectrin or Ig.The DH domain is composed of three structurally conserved regions separated bymore variable regions. It does not share significant sequence homology withother subtypes of small G-protein GEF motifs such as the Cdc25 domain and theSec7 domain, which specifically interact with Ras and ARF family small GTPases, respectively, nor with other Rho protein interactive motifs, indicating that the Dbl family proteins are evolutionarily unique. The DH domain is composed of 11 alpha helices that are folded into a flattened, elongated alpha-helix bundle in which two of the three conserved regions, conserved region 1 (CR1) and conserved region 3 (CR3), are exposed near the centre of one surface. CR1 and CR3, together with a part of alpha-6 and the DH/PH junction site, constitute the Rho GTPase interacting pocket.

Publication Counts Displayer

0 Child Features

1 Contains

DB identifier Name Short Name Type
IPR001331 Guanine-nucleotide dissociation stimulator, CDC24, conserved site GDS_CDC24_CS Conserved_site

5 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF00621 PFAM IPR000219
PS50010 PROFILE IPR000219
SM00325 SMART IPR000219
G3DSA:1.20.900.10 GENE3D IPR000219
SSF48065 SSF IPR000219

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

1 Found In

DB identifier Name Short Name Type
IPR015721 Rho GTP exchange factor RhoGEF-like Family

2 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Dbl homology (DH) domain   GO:0005089 Rho guanyl-nucleotide exchange factor activity
Dbl homology (DH) domain   GO:0035023 regulation of Rho protein signal transduction

2 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Dbl homology (DH) domain   GO:0005089 Rho guanyl-nucleotide exchange factor activity
Dbl homology (DH) domain   GO:0035023 regulation of Rho protein signal transduction

0 Parent Features

378 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0302018 M9PHU0 Drosophila melanogaster
FBpp0309639 M9PEZ9 Drosophila melanogaster
FBpp0309640 Q9VX48 Drosophila melanogaster
FBpp0302021 E1JJN5 Drosophila melanogaster
FBpp0086123 A1ZAN7 Drosophila melanogaster
FBpp0086124 A1ZAN6 Drosophila melanogaster
FBpp0304983 Q9VZ85 Drosophila melanogaster
FBpp0307725 M9PBE8 Drosophila melanogaster
FBpp0307723 A8DZ19 Drosophila melanogaster
FBpp0111798 A8DZ20 Drosophila melanogaster
FBpp0307724 M9PGB1 Drosophila melanogaster
FBpp0291316 C3KGL5 Drosophila melanogaster
FBpp0080888 Q9VIN1 Drosophila melanogaster
FBpp0305901 M9PHJ6 Drosophila melanogaster
FBpp0074448 Q9NHV9 Drosophila melanogaster
FBpp0112228 A8JUQ9 Drosophila melanogaster
FBpp0086135 A1ZAQ4 Drosophila melanogaster
FBpp0086134 Q8MKL4 Drosophila melanogaster
FBpp0305708 E1JJB8 Drosophila melanogaster
FBpp0080821 Q9VIV0 Drosophila melanogaster
FBpp0306076 Q8IRJ3 Drosophila melanogaster
FBpp0072543 Q8IRI4 Drosophila melanogaster
FBpp0072540 Q7KVD1 Drosophila melanogaster
FBpp0089283 Q7YZA0 Drosophila melanogaster
FBpp0072541 Q8IRI5 Drosophila melanogaster
FBpp0306082 E1JHV3 Drosophila melanogaster
FBpp0306081 Q9W0S0 Drosophila melanogaster
FBpp0085412 Q7K4E0 Drosophila melanogaster
FBpp0076595 Q9VS45 Drosophila melanogaster
FBpp0076596 Q8IQ85 Drosophila melanogaster