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Protein Domain : IPR003010

Name  Carbon-nitrogen hydrolase Short Name  C-N_Hydrolase
Type  Domain Description  The carbon-nitrogen hydrolase domain is an around 265-residue domain found innumerous enzymes involved in the reduction of organic nitrogen compounds andammonia production. Based on their sequence similarity and on the reactionsthey catalyse, these enzymes can be classified into functionally distinctgroups including [, ]:Nitrilases (EC 3.5.5.1), which cleave various nitriles into thecorresponding acids and ammonia.Cyanide hydratase (EC 4.2.1.66) of pathogenic fungi, which detoxifies HCNthat is released by their hosts, cyanogenic plants, after injury.Aliphatic amidases (EC 3.5.1.4), which enable prokaryotes to use acetamidesas both carbon and nitrogen source.Beta-alanine synthase (N-carbamoyl-beta-alanine amino hydrolase)(EC 3.5.1.6), which catalyses the last step of pyrimidine catabolism.AdgA (for ammonia-dependent growth) from Rhodobacter species (EC 6.3.5.1).It appears to be essential for using various amino acids as nitrogensources.Biotinidase (EC 3.5.1.12), which catalyses the hydrolysis of biocytin tobiotin and lysine.Pantetheinase (EC 3.5.1.92) (Pantetheine hydrolase) (Vanin), whichhydrolyzes specifically one of the carboamide linkages in D-pantetheine,thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.Apolipoprotein N-acyltransferase (EC 2.3.1.-) (gene lnt), a bacterialenzyme that transfers the fatty acyl group on membrane lipoproteins.Glutamine-dependent NAD(+) synthetase (EC=6.3.5.1), which catalyses the final step in NAD+ synthesis [, ]. The carbon-nitrogen hydrolase domain is characterised by several conservedmotifs, one of which contains a cysteines that is part of the catalytic sitein nitrilases. Another highly conserved motif includes a glutamic acid thatmight also be involved in catalysis [].

Publication Counts Displayer

0 Child Features

2 Contains

DB identifier Name Short Name Type
IPR001110 Uncharacterised protein family UPF0012, conserved site UPF0012_CS Conserved_site
IPR000132 Nitrilase/cyanide hydratase, conserved site Nitrilase/CN_hydratase_CS Conserved_site

4 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF00795 PFAM IPR003010
PS50263 PROFILE IPR003010
G3DSA:3.60.110.10 GENE3D IPR003010
SSF56317 SSF IPR003010

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

4 Found In

DB identifier Name Short Name Type
IPR014445 Glutamine-dependent NAD(+) synthetase Gln-dep_NAD_synthase Family
IPR012101 Biotinidase, eukaryotic Biotinidase_euk Family
IPR016707 Conjugal transfer, TraB, rhizobiales Conjugal_tfr_TraB_rhizob Family
IPR017755 N-carbamoylputrescine amidase N-carbamoylputrescine_amidase Family

2 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Carbon-nitrogen hydrolase   GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
Carbon-nitrogen hydrolase   GO:0006807 nitrogen compound metabolic process

2 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Carbon-nitrogen hydrolase   GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
Carbon-nitrogen hydrolase   GO:0006807 nitrogen compound metabolic process

0 Parent Features

69 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0073676 Q9VYA0 Drosophila melanogaster
FBpp0070845 Q8IRR1 Drosophila melanogaster
FBpp0081276 Q9VI04 Drosophila melanogaster
FBpp0072385 O76464 Drosophila melanogaster
FBpp0070846 P83548 Drosophila melanogaster
FBpp0070844 Q9NFP1 Drosophila melanogaster
FBpp0081507 Q9VHE4 Drosophila melanogaster
FBpp0070843 Q9W430 Drosophila melanogaster
FBpp0274839 Q29GG3 Drosophila pseudoobscura
FBpp0275170 B5DNK8 Drosophila pseudoobscura
FBpp0275059 Q29GG4 Drosophila pseudoobscura
FBpp0282773 Q29BL7 Drosophila pseudoobscura
FBpp0284344 Q29HW0 Drosophila pseudoobscura
FBpp0286468 B5DQY7 Drosophila pseudoobscura
FBpp0301900 I5APK4 Drosophila pseudoobscura
FBpp0173224 B4K4Z1 Drosophila mojavensis
FBpp0160067 B4KCX2 Drosophila mojavensis
FBpp0161823 B4KWR0 Drosophila mojavensis
FBpp0163360 B4L7J5 Drosophila mojavensis
FBpp0165387 B4L6J1 Drosophila mojavensis
FBpp0165388 B4L6I9 Drosophila mojavensis
FBpp0165389 B4L6I8 Drosophila mojavensis
FBpp0165390 B4L6I7 Drosophila mojavensis
FBpp0165391 B4L6I6 Drosophila mojavensis
FBpp0168879 B4KRM5 Drosophila mojavensis
FBpp0233494 B4M2T6 Drosophila virilis
FBpp0227145 B4LHD3 Drosophila virilis
FBpp0228874 B4MBJ2 Drosophila virilis
FBpp0229444 B4MFJ4 Drosophila virilis
FBpp0231079 B4M863 Drosophila virilis