modENCODE |  Help |  blog

Protein Domain : IPR003527

Name  Mitogen-activated protein (MAP) kinase, conserved site Short Name  MAP_kinase_CS
Type  Conserved_site Description  Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function. Phosphoprotein phosphatases catalyse the reverse process. Protein kinases fall into three broad classes, characterised with respect to substrate specificity []:Serine/threonine-protein kinasesTyrosine-protein kinasesDual specificity protein kinases (e.g. MEK - phosphorylates both Thr and Tyr on target proteins)Protein kinase function is evolutionarily conserved from Escherichia coli to human []. Protein kinases play a role in a multitude of cellular processes, including division, proliferation, apoptosis, and differentiation []. Phosphorylation usually results in a functional change of the target protein by changing enzyme activity, cellular location, or association with other proteins. The catalytic subunits of protein kinases are highly conserved, and several structures have been solved [], leading to large screens to develop kinase-specific inhibitors for the treatments of a number of diseases [].Eukaryotic serine-threonine mitogen-activated protein (MAP) kinases are key regulators of cellular signal transduction systems and are conserved from Saccharomyces cerevisiae(Baker's yeast) to human beings. MAPK pathways are signalling cascades differentially regulated by growth factors, mitogens, hormones and stress which mediate cell growth, differentiation and survival. MAPK activity is regulated through a (usually) three-tiered cascade composed of a MAPK, a MAPK kinase (MAPKK, MEK) and a MAPK kinase kinase (MAPKK, MEKK). Substrates for the MAPKs include other kinases and transcription factors []. Mammals express at least four distinctly related groups of MAPKs, extracellularly-regulated kinases (ERKs), c-jun N-terminal kinases (JNKs), p38 proteins and ERK5. Plant MAPK pathways have attracted increasing interest, resulting in the isolation of a large number of different components of MAPK cascades. MAPKs play important roles in the signalling of most plant hormones and in developmental processes []. In the budding yeast S. cerevisiae, four separate but structurally related mitogen-activated protein kinase (MAPK)activation pathways are known, regulating mating, cell integrity and osmosity [].Enzymes in this family are characterised by two domains separated by a deep channel where potential substrates might bind. The N-terminal domain creates a binding pocket for the adenine ring of ATP, and the C-terminal domain contains the catalytic base, magnesium binding sites and phosphorylation lip []. Almost all MAPKs possess a conservedTXY motif in which both the threonine andtyrosine residues are phosphorylated during activation of the enzyme byupstream dual-specificity MAP kinase kinases (MAPKKs).

Publication Counts Displayer

0 Child Features

1 Contains

DB identifier Name Short Name Type
IPR008271 Serine/threonine-protein kinase, active site Ser/Thr_kinase_AS Active_site

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PS01351 PROSITE IPR003527

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

6 Found In

DB identifier Name Short Name Type
IPR011009 Protein kinase-like domain Kinase-like_dom Domain
IPR000719 Protein kinase domain Prot_kinase_dom Domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain Ser/Thr_dual-sp_kinase_dom Domain
IPR008349 Mitogen-activated protein (MAP) kinase, ERK1/2 MAPK_ERK1/2 Family
IPR008351 Mitogen-activated protein (MAP) kinase, JNK MAPK_JNK Family
IPR008352 Mitogen-activated protein (MAP) kinase, p38 MAPK_p38 Family

3 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Mitogen-activated protein (MAP) kinase, conserved site   GO:0004707 MAP kinase activity
Mitogen-activated protein (MAP) kinase, conserved site   GO:0005524 ATP binding
Mitogen-activated protein (MAP) kinase, conserved site   GO:0006468 protein phosphorylation

3 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Mitogen-activated protein (MAP) kinase, conserved site   GO:0004707 MAP kinase activity
Mitogen-activated protein (MAP) kinase, conserved site   GO:0005524 ATP binding
Mitogen-activated protein (MAP) kinase, conserved site   GO:0006468 protein phosphorylation

0 Parent Features

91 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0083967 P83100 Drosophila melanogaster
FBpp0292091 M9MSN4 Drosophila melanogaster
FBpp0292090 Q9W354 Drosophila melanogaster
FBpp0076471 Q8T030 Drosophila melanogaster
FBpp0076473 Q8IQ91 Drosophila melanogaster
FBpp0079676 P92208 Drosophila melanogaster
FBpp0112426 P40417 Drosophila melanogaster
FBpp0083965 O62618 Drosophila melanogaster
FBpp0080111 O61443 Drosophila melanogaster
FBpp0281996 Q298J6 Drosophila pseudoobscura
FBpp0273614 Q29IC3 Drosophila pseudoobscura
FBpp0281997 B5DVL2 Drosophila pseudoobscura
FBpp0287310 Q29K65 Drosophila pseudoobscura
FBpp0159856 B4KHP0 Drosophila mojavensis
FBpp0162365 B4KUQ0 Drosophila mojavensis
FBpp0167449 B4KIW9 Drosophila mojavensis
FBpp0167476 B4KJ18 Drosophila mojavensis
FBpp0172645 B4K7E5 Drosophila mojavensis
FBpp0228314 B4LHS8 Drosophila virilis
FBpp0228948 B4MBY2 Drosophila virilis
FBpp0231935 B4LQZ8 Drosophila virilis
FBpp0232557 B4M8K4 Drosophila virilis
FBpp0233732 B4M1I9 Drosophila virilis
FBpp0236301 B4LR19 Drosophila virilis
FBpp0255834 B4PL11 Drosophila yakuba
FBpp0256337 B4IST6 Drosophila yakuba
FBpp0260469 B4PWU9 Drosophila yakuba
A8XZI6_CAEBR A8XZI6 Caenorhabditis briggsae
A8X1S0_CAEBR A8X1S0 Caenorhabditis briggsae
A8XI46_CAEBR A8XI46 Caenorhabditis briggsae