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Protein Domain : IPR003619

Name  MAD homology 1, Dwarfin-type Short Name  MAD_homology1_Dwarfin-type
Type  Domain Description  Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth []. The dwarfin family also includes the Drosophila protein MAD that is required for the function of decapentaplegic (DPP) and may play a role in DPP signalling. Drosophila Mad binds to DNA and directly mediates activation of vestigial by Dpp []. This domain is also found in nuclear factor I (NF-I) or CCAAT box-binding transcription factor (CTF).This entry represents the MH1 (MAD homology 1) domain is found at the amino terminus of MAD related proteins such as Smads. This domain is separated from the MH2 domain by a non-conserved linker region. The crystal structure of the MH1 domain shows that a highly conserved 11 residue beta hairpin is used to bind the DNA consensus sequence GNCN in the major groove, shown to be vital for the transcriptional activation of target genes. Not all examples of MH1 can bind to DNA however. Smad2 cannot bind DNA and has a large insertion within the hairpin that presumably abolishes DNA binding. A basic helix (H2) in MH1 with the nuclear localisation signal KKLKK has been shown to be essential for Smad3 nuclear import. Smads also use the MH1 domain to interact with transcription factors such as Jun, TFE3, Sp1, and Runx [, , ].

Publication Counts Displayer

1 Child Features

DB identifier Name Short Name Type
IPR013019 MAD homology, MH1 MAD_homology_MH1 Domain

0 Contains

2 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF03165 PFAM IPR003619
SM00523 SMART IPR003619

3 Data Sets

Name URL
TrEMBL data set http://www.ebi.ac.uk/trembl/
InterPro data set  
InterPro GO Annotation data set  

2 Found In

DB identifier Name Short Name Type
IPR000647 CTF transcription factor/nuclear factor 1 CTF/NFI Family
IPR020604 CTF transcription factor/nuclear factor 1, DNA-binding domain CTF/NFI_DNA-bd-dom Domain

2 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
MAD homology 1, Dwarfin-type   GO:0006355 regulation of transcription, DNA-templated
MAD homology 1, Dwarfin-type   GO:0005622 intracellular

2 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
MAD homology 1, Dwarfin-type   GO:0006355 regulation of transcription, DNA-templated
MAD homology 1, Dwarfin-type   GO:0005622 intracellular

0 Parent Features

75 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0099512 Q5U110 Drosophila melanogaster
FBpp0085176 O62609 Drosophila melanogaster
FBpp0085177 Q8IMG6 Drosophila melanogaster
FBpp0077302 P42003 Drosophila melanogaster
FBpp0071140 O96660 Drosophila melanogaster
FBpp0088208 Q86P06 Drosophila melanogaster
FBpp0304648 M9PBX0 Drosophila melanogaster
FBpp0305834 L0MPW8 Drosophila melanogaster
FBpp0297040 I5AND6 Drosophila pseudoobscura
FBpp0297039 Q29AF0 Drosophila pseudoobscura
FBpp0282725 Q29HD9 Drosophila pseudoobscura
FBpp0284200 Q297H8 Drosophila pseudoobscura
FBpp0287731 B5DJI3 Drosophila pseudoobscura
FBpp0163324 B4L7E0 Drosophila mojavensis
FBpp0163632 B4L8H6 Drosophila mojavensis
FBpp0167198 B4KEV0 Drosophila mojavensis
FBpp0172617 B4K7Y4 Drosophila mojavensis
FBpp0173071 B4KC87 Drosophila mojavensis
FBpp0224986 B4M5J3 Drosophila virilis
FBpp0233179 B4M1Z3 Drosophila virilis
FBpp0233970 B4LU30 Drosophila virilis
FBpp0235751 B4MEX9 Drosophila virilis
FBpp0237509 B4M0Z7 Drosophila virilis
FBpp0255962 B4PNF5 Drosophila yakuba
FBpp0259530 B4PVZ8 Drosophila yakuba
FBpp0259945 B4NXE4 Drosophila yakuba
FBpp0262489 B4Q0M2 Drosophila yakuba
FBpp0269616 B4PM83 Drosophila yakuba
A8XPG1_CAEBR A8XPG1 Caenorhabditis briggsae
A8X5U1_CAEBR A8X5U1 Caenorhabditis briggsae