modENCODE |  Help |  blog

Protein Domain : IPR002906

Name  Ribosomal protein S27a Short Name  Ribosomal_S27a
Type  Domain Description  Ribosomes are the particles that catalyse mRNA-directed protein synthesis in all organisms. The codons of the mRNA are exposed on the ribosome to allow tRNA binding. This leads to the incorporation of amino acids into the growing polypeptide chain in accordance with the genetic information. Incoming amino acid monomers enter the ribosomal A site in the form of aminoacyl-tRNAs complexed with elongation factor Tu (EF-Tu) and GTP. The growing polypeptide chain, situated in the P site as peptidyl-tRNA, is then transferred to aminoacyl-tRNA and the new peptidyl-tRNA, extended by one residue, is translocated to the P site with the aid the elongation factor G (EF-G) and GTP as the deacylated tRNA is released from the ribosome through one or more exit sites [, ]. About 2/3 of the mass of the ribosome consists of RNA and 1/3 of protein. The proteins are named in accordance with the subunit of the ribosome which they belong to - the small (S1 to S31) and the large (L1 to L44). Usually they decorate the rRNA cores of the subunits. Many ribosomal proteins, particularly those of the large subunit, are composed of a globular, surfaced-exposed domain with long finger-like projections that extend into the rRNA core to stabilise its structure. Most of the proteins interact with multiple RNA elements, often from different domains. In the large subunit, about 1/3 of the 23S rRNA nucleotides are at least in van der Waal's contact with protein, and L22 interacts with all six domains of the 23S rRNA. Proteins S4 and S7, which initiate assembly of the 16S rRNA, are located at junctions of five and four RNA helices, respectively. In this way proteins serve to organise and stabilise the rRNA tertiary structure. While the crucial activities of decoding and peptide transfer are RNA based, proteins play an active role in functions that may have evolved to streamline the process of protein synthesis. In addition to their function in the ribosome, many ribosomal proteins have some function 'outside' the ribosome [, ].This family of ribosomal proteins consists mainly of the 40S ribosomal protein S27a which is synthesized as a C-terminal extension of ubiquitin (CEP) (). The S27adomain compromises the C-terminal half of the protein.The synthesis of ribosomal proteins as extensions of ubiquitin promotes their incorporation into nascent ribosomes by a transient metabolic stabilisation and is required for efficient ribosome biogenesis []. The ribosomal extension protein S27a contains a basic region that is proposed to form a zinc finger; its fusion gene is proposed as a mechanism to maintain a fixed ratio between ubiquitin necessary for degrading proteins and ribosomes asource of proteins [].

Publication Counts Displayer

0 Child Features

0 Contains

1 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF01599 PFAM IPR002906

3 Data Sets

Name URL
Swiss-Prot data set http://ca.expasy.org/sprot
InterPro data set  
InterPro GO Annotation data set  

0 Found In

4 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Ribosomal protein S27a   GO:0003735 structural constituent of ribosome
Ribosomal protein S27a   GO:0006412 translation
Ribosomal protein S27a   GO:0005622 intracellular
Ribosomal protein S27a   GO:0005840 ribosome

4 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Ribosomal protein S27a   GO:0003735 structural constituent of ribosome
Ribosomal protein S27a   GO:0006412 translation
Ribosomal protein S27a   GO:0005622 intracellular
Ribosomal protein S27a   GO:0005840 ribosome

1 Parent Features

DB identifier Name Short Name Type
IPR011332 Zinc-binding ribosomal protein Ribosomal_zn-bd Domain

10 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0079606 P15357 Drosophila melanogaster
FBpp0276130 B5DVA8 Drosophila pseudoobscura
FBpp0159835 B4KHP5 Drosophila mojavensis
RS27A_CAEBR P37164 Caenorhabditis briggsae
B4LR26_DROVI B4LR26 Drosophila virilis
H2WZ45_CAEJA H2WZ45 Caenorhabditis japonica
G0MWR8_CAEBE G0MWR8 Caenorhabditis brenneri
RS27A_CAEEL P37165 Caenorhabditis elegans
RS27A_CAEEL-2 P37165-2 Caenorhabditis elegans
E3MWD1_CAERE E3MWD1 Caenorhabditis remanei