modENCODE |  Help |  blog

Protein Domain : IPR005475

Name  Transketolase-like, pyrimidine-binding domain Short Name  Transketolase-like_Pyr-bd
Type  Domain Description  Transketolase (TK) catalyzes the reversible transfer of atwo-carbon ketol unit from xylulose 5-phosphate to an aldose receptor, such asribose 5-phosphate, to form sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate. This enzyme, together with transaldolase, provides a link betweenthe glycolytic and pentose-phosphate pathways.TK requires thiamine pyrophosphate as a cofactor. In most sources where TK hasbeen purified, it is a homodimer of approximately 70 Kd subunits. TK sequencesfrom a variety of eukaryotic and prokaryotic sources [, ]show that theenzyme has been evolutionarily conserved.In the peroxisomes of methylotrophic yeast Pichia angusta(Yeast) (Hansenula polymorpha), there is ahighly related enzyme, dihydroxy-acetone synthase (DHAS) (alsoknown as formaldehyde transketolase), which exhibits a very unusualspecificity by including formaldehyde amongst its substrates.1-deoxyxylulose-5-phosphate synthase (DXP synthase) []is an enzyme so farfound in bacteria (gene dxs) and plants (gene CLA1) which catalyzes thethiamine pyrophosphoate-dependent acyloin condensation reaction between carbonatoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (dxp), a precursor in the biosynthetic pathway toisoprenoids, thiamine (vitamin B1), and pyridoxol (vitamin B6). DXP synthaseis evolutionary related to TK. The N-terminal section, contains a histidine residue which appears to function inproton transfer during catalysis []. In the centralsection there are conserved acidic residues that are part of the active cleftand may participate in substrate-binding [].This family includes transketolase enzymes and also partially matches to 2-oxoisovalerate dehydrogenasebeta subunit . Both these enzymesutilise thiamine pyrophosphate as a cofactor, suggestingthere may be common aspects in their mechanism of catalysis.

Publication Counts Displayer

0 Child Features

1 Contains

DB identifier Name Short Name Type
IPR020826 Transketolase binding site Transketolase_BS Binding_site

2 Cross References

Source . Name

Subject . Primary Identifier
PF02779 PFAM IPR005475
SM00861 SMART IPR005475

2 Data Sets

Name URL
Swiss-Prot data set
InterPro data set  

3 Found In

DB identifier Name Short Name Type
IPR011603 2-oxoglutarate dehydrogenase, E1 component 2oxoglutarate_DH_E1 Family
IPR005478 Transketolase, bacterial-like Transketolase_bac-like Family
IPR005477 Deoxyxylulose-5-phosphate synthase Dxylulose-5-P_synthase Family

0 GO Annotation

0 Ontology Annotations

0 Parent Features

79 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0075028 Q9VVC5 Drosophila melanogaster
FBpp0072631 Q95T35 Drosophila melanogaster
FBpp0072633 Q0E8J6 Drosophila melanogaster
FBpp0075027 Q8IQQ0 Drosophila melanogaster
FBpp0291602 A8QHW8 Drosophila melanogaster
FBpp0291601 Q7PLE6 Drosophila melanogaster
FBpp0084735 Q7K5K3 Drosophila melanogaster
FBpp0084737 Q8IML6 Drosophila melanogaster
FBpp0074898 Q9VVP4 Drosophila melanogaster
FBpp0081370 Q9VHN7 Drosophila melanogaster
FBpp0081372 Q7KSU6 Drosophila melanogaster
FBpp0085117 Q9VA02 Drosophila melanogaster
FBpp0290113 E1JHW5 Drosophila melanogaster
FBpp0111773 A8JNU6 Drosophila melanogaster
FBpp0288163 B5DSC2 Drosophila pseudoobscura
FBpp0281830 B5DWE4 Drosophila pseudoobscura
FBpp0293043 I5AMT5 Drosophila pseudoobscura
FBpp0282990 Q29CE4 Drosophila pseudoobscura
FBpp0286887 Q29E02 Drosophila pseudoobscura
FBpp0286941 Q29DU3 Drosophila pseudoobscura
FBpp0283602 Q29AC1 Drosophila pseudoobscura
FBpp0292736 I5ANG4 Drosophila pseudoobscura
FBpp0161685 B4KYN6 Drosophila mojavensis
FBpp0162486 B4KW84 Drosophila mojavensis
FBpp0167472 B4KJ09 Drosophila mojavensis
FBpp0171488 B4K754 Drosophila mojavensis
FBpp0171538 B4K6G8 Drosophila mojavensis
FBpp0171750 B4KCP2 Drosophila mojavensis
FBpp0224553 B4M501 Drosophila virilis
FBpp0224925 B4M5V2 Drosophila virilis