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Protein Domain : IPR001506

Name  Peptidase M12A, astacin Short Name  Peptidase_M12A
Type  Domain Description  This group of metallopeptidases belong to the MEROPS peptidase family M12, subfamily M12A (astacin family, clan MA(M)). The protein fold of the peptidase domain for members of this family resembles that of thermolysin, the type example for clan MA and the predicted active site residues for members of this family and thermolysin occur in the motif HEXXH [].The astacin () family of metalloendopeptidases encompasses a range of proteinsfound in hydra to humans, in mature and developmental systems []. Theirfunctions include activation of growth factors, degradation of polypeptides,and processing of extracellular proteins []. The proteins are synthesisedwith N-terminal signal and pro-enzyme sequences, and many contain multipledomains C-terminalto the protease domain. They are either secreted fromcells, or are associated with the plasma membrane.The astacin molecule adopts a kidney shape, with a deep active-site cleftbetween its N- and C-terminal domains []. The zinc ion, which lies at thebottom of the cleft, exhibits a unique penta-coordinated mode of binding,involving 3 histidine residues, a tyrosine and a water molecule (which isalso bound to the carboxylate side chain of Glu93) []. The N-terminaldomain comprises 2 alpha-helices and a 5-stranded beta-sheet. The overalltopology of this domain is shared by the archetypal zinc-endopeptidasethermolysin. Astacin protease domains also share common features withserralysins, matrix metalloendopeptidases, and snake venom proteases; theycleave peptide bonds in polypeptides such as insulin B chain and bradykinin,and in proteins such as casein and gelatin; and they have arylamidaseactivity [].

Publication Counts Displayer

0 Child Features

0 Contains

2 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF01400 PFAM IPR001506
PR00480 PRINTS IPR001506

3 Data Sets

Name URL
Swiss-Prot data set http://ca.expasy.org/sprot
InterPro data set  
InterPro GO Annotation data set  

7 Found In

DB identifier Name Short Name Type
IPR017050 Peptidase M12A, astacin, nematode Peptidase_M12A_nem Family
IPR017367 Peptidase M12A, nematode astacin 7/8 Peptidase_M12A_astacin-7/8 Family
IPR017368 Peptidase M12A, nematode astacin 9/10/11 Peptidase_M12A_astacin-9/10/11 Family
IPR015446 Bone morphogenetic protein 1/tolloid-like protein BMP_1/tolloid-like Family
IPR017369 SPAN protein/blastula protease 10 SPAN/blastula_protease_10 Family
IPR017370 Hatching enzyme, Uvs2 Hatching_enzyme_Uvs2 Family
IPR008294 Meprin alpha/beta subunit Meprin Family

2 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Peptidase M12A, astacin   GO:0004222 metalloendopeptidase activity
Peptidase M12A, astacin   GO:0006508 proteolysis

2 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Peptidase M12A, astacin   GO:0004222 metalloendopeptidase activity
Peptidase M12A, astacin   GO:0006508 proteolysis

1 Parent Features

DB identifier Name Short Name Type
IPR006026 Peptidase, metallopeptidase Peptidase_Metallo Domain

309 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0078297 Q9VNK5 Drosophila melanogaster
FBpp0080342 Q9VJN9 Drosophila melanogaster
FBpp0082501 Q9VFD6 Drosophila melanogaster
FBpp0074382 Q9VWR6 Drosophila melanogaster
FBpp0083770 Q9VCN5 Drosophila melanogaster
FBpp0084069 Q9VC47 Drosophila melanogaster
FBpp0080340 Q9V421 Drosophila melanogaster
FBpp0084018 Q9VC98 Drosophila melanogaster
FBpp0080343 Q9V445 Drosophila melanogaster
FBpp0080312 Q9V402 Drosophila melanogaster
FBpp0080313 Q9V3R0 Drosophila melanogaster
FBpp0080341 Q9V3M3 Drosophila melanogaster
FBpp0084070 P25723 Drosophila melanogaster
FBpp0287786 Q29KV7 Drosophila pseudoobscura
FBpp0272266 Q29FZ0 Drosophila pseudoobscura
FBpp0281582 Q296J1 Drosophila pseudoobscura
FBpp0282336 Q29A67 Drosophila pseudoobscura
FBpp0282875 Q29BZ7 Drosophila pseudoobscura
FBpp0282876 B5DXS7 Drosophila pseudoobscura
FBpp0284091 Q297X5 Drosophila pseudoobscura
FBpp0284249 Q297B8 Drosophila pseudoobscura
FBpp0287528 Q29KV9 Drosophila pseudoobscura
FBpp0287527 Q29KV8 Drosophila pseudoobscura
FBpp0287529 B5DJL4 Drosophila pseudoobscura
FBpp0287787 Q29KV6 Drosophila pseudoobscura
FBpp0287788 Q29KV5 Drosophila pseudoobscura
FBpp0287790 Q29KV4 Drosophila pseudoobscura
FBpp0160150 B4L8D4 Drosophila mojavensis
FBpp0173585 B4KAV9 Drosophila mojavensis
FBpp0167121 B4KLI2 Drosophila mojavensis