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Protein Domain : IPR007087

Name  Zinc finger, C2H2 Short Name  Znf_C2H2
Type  Domain Description  Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not; instead binding other metals such as iron, or no metal at all. For example, some family members form salt bridges to stabilise the finger-like folds. They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis(African clawed frog), however they are now recognised to bind DNA, RNA, protein and/or lipid substrates [, , , , ]. Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. Znf domains are often found in clusters, where fingers can have different binding specificities. There are many superfamilies of Znf motifs, varying in both sequence and structure. They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. For example, Znf-containing proteins function in gene transcription, translation, mRNA trafficking, cytoskeleton organisation, epithelial development, cell adhesion, protein folding, chromatin remodelling and zinc sensing, to name but a few []. Zinc-binding motifs are stable structures, and they rarely undergo conformational changes upon binding their target. The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger: #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C], where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter [].This entry represents the classical C2H2 zinc finger domain.

Publication Counts Displayer

0 Child Features

1 Contains

DB identifier Name Short Name Type
IPR015318 Zinc finger, GAGA-binding factor Znf_GAGA-bd_fac Domain

3 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF00096 PFAM IPR007087
PS00028 PROSITE IPR007087
PS50157 PROFILE IPR007087

3 Data Sets

Name URL
Swiss-Prot data set http://ca.expasy.org/sprot
InterPro data set  
InterPro GO Annotation data set  

14 Found In

DB identifier Name Short Name Type
IPR007042 Arsenite-resistance protein 2 Arsenite-R_2 Domain
IPR013087 Zinc finger C2H2-type/integrase DNA-binding domain Znf_C2H2/integrase_DNA-bd Domain
IPR004595 TFIIH C1-like domain TFIIH_C1-like_dom Domain
IPR016608 PR-domain zinc finger protein PRDM1 Znf_PRDM1 Family
IPR009170 Predicted retinoblastoma binding protein (RIZ) RIZ_retinblastoma-bd_prot Family
IPR017126 PR-domain zinc finger protein PRDM12 Znf_PRDM12 Family
IPR017125 PR-domain zinc finger protein PRDM5 Znf_PRDM5 Family
IPR017124 PR-domain zinc finger protein PRDM4 Znf_PRDM4 Family
IPR017114 Transcription factor yin/yang TF_Yin_yang Family
IPR017083 RNA editing complex, nuclease subunit MP42 RNA_edit_cplx_Nase-su_MP42 Family
IPR017086 RNA editing complex, structural subunit MP63 RNA_edit_cplx_Nase-su_MP63 Family
IPR017085 RNA editing complex, structural subunit MP81 RNA_edit_cplx_Nase-su_MP81 Family
IPR016766 Transcription regulator Tri6 Tscrpt_reg_Tri6 Family
IPR016378 Transcription factor, cyclic AMP-dependent TF_cAMP-dep Family

1 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Zinc finger, C2H2   GO:0046872 metal ion binding

1 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
Zinc finger, C2H2   GO:0046872 metal ion binding

1 Parent Features

DB identifier Name Short Name Type
IPR015880 Zinc finger, C2H2-like Znf_C2H2-like Domain

3298 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0088052 Q1LZ24 Drosophila melanogaster
FBpp0087331 Q7JW60 Drosophila melanogaster
FBpp0077247 Q9VQS7 Drosophila melanogaster
FBpp0087330 Q7JQY8 Drosophila melanogaster
FBpp0099541 Q7KAH0 Drosophila melanogaster
FBpp0305260 M9PFM7 Drosophila melanogaster
FBpp0099866 A4V1Y7 Drosophila melanogaster
FBpp0089417 A4V1Y6 Drosophila melanogaster
FBpp0305259 Q2PDY1 Drosophila melanogaster
FBpp0099867 Q2PDY2 Drosophila melanogaster
FBpp0074392 Q9VWT2 Drosophila melanogaster
FBpp0073022 Q9VZK2 Drosophila melanogaster
FBpp0081613 Q9VH70 Drosophila melanogaster
FBpp0081832 Q9VGQ5 Drosophila melanogaster
FBpp0288841 Q7KSP9 Drosophila melanogaster
FBpp0078205 Q9VNP8 Drosophila melanogaster
FBpp0081490 Q9VHJ3 Drosophila melanogaster
FBpp0073882 Q9VXT1 Drosophila melanogaster
FBpp0301059 R9PY46 Drosophila melanogaster
FBpp0290458 A8JV12 Drosophila melanogaster
FBpp0290459 A8JV11 Drosophila melanogaster
FBpp0072245 Q9W137 Drosophila melanogaster
FBpp0292193 S0ARU4 Drosophila melanogaster
FBpp0070870 Q9W403 Drosophila melanogaster
FBpp0085079 Q86PF2 Drosophila melanogaster
FBpp0110305 Q7YU81 Drosophila melanogaster
FBpp0072351 P07247 Drosophila melanogaster
FBpp0072808 Q0E8J0 Drosophila melanogaster
FBpp0080774 Q9VIY9 Drosophila melanogaster
FBpp0099852 Q59E05 Drosophila melanogaster