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Protein Domain : IPR000182

Name  GNAT domain Short Name  GNAT_dom
Type  Domain Description  The N-acetyltransferases (NAT) (EC 2.3.1.-) are enzymes that use acetylcoenzyme A (CoA) to transfer an acetyl group to a substrate, a reactionimplicated in various functions from bacterial antibiotic resistance tomammalian circadian rhythm and chromatin remodeling. The Gcn5-relatedN-acetyltransferases (GNAT) catalyze the transfer of the acetyl from the CoAdonor to a primary amine of the acceptor. The GNAT proteins share a domaincomposed of four conserved sequence motifs A-D [, ]. This GNAT domain is named after yeast GCN5 (from General Control Nonrepressed) and related histone acetyltransferases (HATs) like Hat1 and PCAF. HATs acetylate lysine residues of amino terminal histone tails, resulting in transcription activation. Another category of GNAT, the aminoglycoside N-acetyltransferases, confer antibiotic resistance by catalyzing the acetylation of amino groups in aminoglycoside antibiotics []. GNAT proteins can also have anabolic and catabolic functions in both prokaryotes and eukaryotes [, , , , ].The acetyltransferase/GNAT domain forms a structurally conserved fold of 6 to7 beta strands (B) and 4 helices (H) in the topology B1-H1-H2-B2-B3-B4-H3-B5-H4-B6, followed by a C-terminal strand which may be from the same monomer or contributed by another [, ]. Motifs D (B2-B3), A (B4-H3) and B (B5-H4) are collectively called the HAT core[, , ], while the N-terminal motif C (B1-H1) is less conserved.Some proteins known to contain a GNAT domain:Yeast GCN5 and Hat1, which are histone acetyltransferases (EC 2.3.1.48).Human PCAF, a histone acetyltransferase.Mammalian serotonin N-acetyltransferase (SNAT) or arylalkylamine NAT(AANAT), which acetylates serotonin into a circadian neurohormone that mayparticipate in light-dark rhythms, and human mood and behavior.Mammalian glucosamine 6-phosphate N-acetyltransferase (GNA1) (EC 2.3.1.4).Escherichia coli rimI and rimJ, which acetylate the N-terminal alanine ofribosomal proteins S18 and S5, respectively (EC 2.3.1.128).Mycobacterium tuberculosis aminoglycoside 2'-N-acetyltransferase (aac),which acetylates the 2' hydroxyl or amino group of a broad spectrum ofaminoglycoside antibiotics.Bacillus subtilis bltD and paiA, which acetylate spermine and spermidine.This entry represents the entire GNAT domain.

Publication Counts Displayer

0 Child Features

0 Contains

6 Cross References

Identifier
Source . Name

Subject . Primary Identifier
PF00583 PFAM IPR000182
PF13302 PFAM IPR000182
PF13508 PFAM IPR000182
PF13673 PFAM IPR000182
PF13718 PFAM IPR000182
PS51186 PROFILE IPR000182

3 Data Sets

Name URL
Swiss-Prot data set http://ca.expasy.org/sprot
InterPro data set  
InterPro GO Annotation data set  

14 Found In

DB identifier Name Short Name Type
IPR005910 Histone acetyltransferase ELP3 Hist_AcTrfase_ELP3 Family
IPR016376 Histone acetylase PCAF Hist_acetylase_PCAF Family
IPR011244 Bifunctional argininosuccinate lyase/acetyltransferase ASAL_AGS_AcTrfase Family
IPR017534 GNAT-acetyltransferase GNAT-acetyltransferase Family
IPR005216 Citrate lyase ligase Citrate_lyase_ligase Family
IPR017813 Mycothiol acetyltransferase Mycothiol_AcTrfase Family
IPR010167 Amino-acid N-acetyltransferase (ArgA) NH2A_AcTrfase_ArgA Family
IPR017274 N-acetyltransferase, YlbP, predicted N-AcTrfase_YlbP_prd Family
IPR017255 Acetyltransferase, GNAT, predicted AcTrfase_GNAT_prd Family
IPR008125 Streptothricin acetyltransferase Streptothricin_AcTrfase Family
IPR012752 TDP-D-fucosamine acetyltransferase WecD AcTrfase_WecD Family
IPR012772 L-2,4-diaminobutyric acid acetyltransferase Ectoine_EctA Family
IPR016890 Uncharacterised conserved protein UCP028520 UCP028520 Family
IPR016794 Uncharacterised conserved protein UCP21603, acetyltransferase-like UCP21603_acetyltransf Family

1 GO Annotation


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
GNAT domain   GO:0008080 N-acetyltransferase activity

1 Ontology Annotations


Subject . Secondary Identifier

Subject . Name

Subject . Symbol

Ontology Term . Identifier

Ontology Term . Name
GNAT domain   GO:0008080 N-acetyltransferase activity

1 Parent Features

DB identifier Name Short Name Type
IPR016181 Acyl-CoA N-acyltransferase Acyl_CoA_acyltransferase Domain

317 Proteins

DB identifier Primary Accession
Organism . Name
FBpp0080882 Q9VIQ1 Drosophila melanogaster
FBpp0099670 Q9VS50 Drosophila melanogaster
FBpp0304758 M9PI14 Drosophila melanogaster
FBpp0304759 Q9VWF5 Drosophila melanogaster
FBpp0071217 Q9W3C1 Drosophila melanogaster
FBpp0080591 Q9VJA0 Drosophila melanogaster
FBpp0099421 Q2PDV0 Drosophila melanogaster
FBpp0289985 Q9VLU3 Drosophila melanogaster
FBpp0100038 Q59DX8 Drosophila melanogaster
FBpp0080602 Q9VJ98 Drosophila melanogaster
FBpp0089101 Q94521 Drosophila melanogaster
FBpp0082508 Q8MRT7 Drosophila melanogaster
FBpp0085227 Q9V9V9 Drosophila melanogaster
FBpp0080059 Q8IP77 Drosophila melanogaster
FBpp0078873 Q9VMG0 Drosophila melanogaster
FBpp0081612 Q9VH73 Drosophila melanogaster
FBpp0084827 Q9VAI4 Drosophila melanogaster
FBpp0087227 Q9NHD5 Drosophila melanogaster
FBpp0072598 Q8IRH2 Drosophila melanogaster
FBpp0085702 A1ZBI9 Drosophila melanogaster
FBpp0084841 Q9VAI0 Drosophila melanogaster
FBpp0077076 Q9VR30 Drosophila melanogaster
FBpp0084840 Q8IMK5 Drosophila melanogaster
FBpp0081086 Q8INT0 Drosophila melanogaster
FBpp0070202 Q95RC0 Drosophila melanogaster
FBpp0070797 Q9W469 Drosophila melanogaster
FBpp0080033 Q8IP78 Drosophila melanogaster
FBpp0089006 Q95SX8 Drosophila melanogaster
FBpp0075701 Q9VTZ1 Drosophila melanogaster
FBpp0077129 Q9VQZ6 Drosophila melanogaster