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Unstranded Cell Line Transcriptional Profiling Using Illumina poly(A)+ RNA-seq

experiment: Unstranded Cell Line Transcriptional Profiling Using Illumina poly(A)+ RNA-seq project: The Drosophila Transcriptome PI: Sue Celniker Labs: Brenton Graveley
We are using Paired-end RNA-Seq to characterize the transcriptome in various Drosophila cell lines. For each cell line, total RNA is isolated, subjected to two rounds of poly(A)+ selection, and then used to make paired-end RNA-Seq libraries. We then sequence each library using an Illumina Genome Analyzer.