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experiment: Tissue-specific Poly(A) Site Profiling of D. melanogaster using Illumina poly(A)+ RNA-Seq | project: The Drosophila Transcriptome | PI: Sue Celniker | Labs: Brenton Graveley |
| RNA-seq analysis was performed on poly(A)-site enriched RNA from various tissues dissected from various stages of D. melanogaster. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A)+ RNA, strand-specific library construction, poly(A)-site enrichment and paired end (2X100, plus an index read) sequencing on an Illumina HiSeq 2000 were performed in the Brenton Graveley lab. Fastq files were generated using CASAVA. | ||||
