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Small RNA expression in C. elegans embryos

experiment: Small RNA expression in C. elegans embryos project: The 3' UTRome PI: Fabio Piano Labs: Nikolaus Rajewsky
We devised a method to collect various staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). We combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We aim to discover complex changes in the expression between and within almost all classes of small rnAs, including micrornAs and 26G-RNAs, during embryogenesis.