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MacAlpine Early Origin of Replication Identification

experiment: MacAlpine Early Origin of Replication Identification project: Origins of Replication PI: David MacAlpine Labs: David MacAlpine
Early origins of replication were identified by treating cells with hydroxyurea (HU), a potent inhibitor of nucleotide synthesis, in the presence of the nucleotide analogue BrdU. Treatment of synchronized cells with HU stalls replication forks and activates the intra S-phase checkpoint, thereby limiting BrdU incorporation to those sequences immediately adjacent to early activating replication origins. BrdU enriched sequences surrounding early origins of replication are then enriched by immunoprecipitation with an anti-BrdU antibody. Early origins are then detected by hybridization to Agilent genomic tiling arrays.

All features generated by this experiment:
Feature Export
OriginOfReplication ? 1520
This represents 1514 unique OriginOfReplications
TAB CSV GFF3 (for UCSC) SEQ
All GBrowse tracks generated for this experiment:
GBrowse tracks: by chromosome
There are 3 tiling array: DNA submissions for this experiment:
DCC id Name cell line Embargoed until Features, GBrowse and Data Files
modENCODE_709


Related to: modENCODE_3441

Kc167 Replication Origins Kc167 [ALL]
embargo expired
? OriginOfReplication: 630   export: TAB   CSV  GFF3  (for UCSC)  SEQ    create LIST
  [find nearby features]
GBrowse tracks: Kc


10 files Download data files
modENCODE_711


Related to: modENCODE_3441

Bg3 Replication Origins ML-DmBG3-c2 [ALL]
embargo expired
? OriginOfReplication: 433   export: TAB   CSV  GFF3  (for UCSC)  SEQ    create LIST
  [find nearby features]
GBrowse tracks: Bg3


8 files Download data files
modENCODE_710


Related to: modENCODE_3441

S2 Replication Origins S2-DRSC [ALL]
embargo expired
? OriginOfReplication: 457   export: TAB   CSV  GFF3  (for UCSC)  SEQ    create LIST
  [find nearby features]
GBrowse tracks: S2


8 files Download data files