RNA-seq analysis onthe 454 platform was performed on poly(A)+ RNA from selected developmental stages spanning the life cycle of D. melanogaster. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A)+ RNA and library construction was performed by Blanchette, Cherbas, or Roche. Libraries were sequenced at Roche or in the Blanchette lab. SFF files were generated and submitted to SRA.
