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experiment: Developmental Time Course Transcriptional Profiling of D. melanogaster Using Illumina poly(A)+ RNA-Seq SPA+Bowtie analysis | project: The Drosophila Transcriptome | PI: Sue Celniker | Labs: Brenton Graveley |
RNA-seq analysis was performed on poly(A)+ RNA from 30 developmental stages spanning the life cycle of D. melanogaster, from 0-2hr embryos through 30-day male and female adults. Unstranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011) |